Effects of weight loss on adipose tissue β2-adrenoceptor-stimulated lipolysis: role of adipose tissue oxidative stress and hypoxia
Completed
- Conditions
- vetweefseldisfunctie bij obesitasobesity
- Registration Number
- NL-OMON36127
- Lead Sponsor
- niversiteit Maastricht
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 37
Inclusion Criteria
- All subjects entering the CO-Eur programme (life style intervention at the obesity treatment centre
CO-Eur in Heerlen). Co-Eur only includes subjects with age>18 years and BMI>30kg/m2
- No more than 3 kg weight change in the past 3 months
- age>18 years
Exclusion Criteria
- Smoking
* Diabetes
* (Medicinal) use of β-blockers or β-agonists
* Pregnant women
- (medicinal) use of anticoagulantia or blood clotting problems
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>For the pre- and post-measurement a subcutaneous adipose tissue biopsy of about<br /><br>1 gram will be taken. one part will be used to measure sensitivity to β2-AR<br /><br>stimulation of lipolysis with salbutamol in adipocytes isolated from the<br /><br>biopsy.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Another part of the adipose tissue biopsy will be used to measure markers of<br /><br>local adipose tissue hypoxia and oxidative stress with western blot and PCR<br /><br>techniques.<br /><br>In addition a 5 ml venous blood sample and a small urine sample will be<br /><br>collected at both occasions. Markers of systemic oxidative stress will be<br /><br>measured in plasma and urine samples.</p><br>