Effects of Fasting and Exercise on Insulin and GH Signaltransduction in Muscle and Fat
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Aarhus
- Enrollment
- 8
- Locations
- 1
- Primary Endpoint
- Growth hormone and insulin signal pathways
Study Overview
Brief Summary
Hypothesis: under fasting there will primarily be an activation of growth hormone(GH) signalpathways and a inhibition of insulin signalpathways, and the opposite will happen under physical activity.
Primarily we will investigate markers for GH and insulin signaltransduction in muscle- and fat-biopsies after fasting for 72 hours and after 1 hour of ergometer-cycling at 65% Vo2max.
In addition to this we will investigate the effect of fasting and exercise on the velocity of carbamide synthesis, aminoacid rate of turnover and glucose rate of turnover.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 45 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •healthy men
- •Body mass index (BMI) 20-26
- •age 18-45 years
Exclusion Criteria
- •any kind of disease
- •any use of medicine
Arms & Interventions
Exercise
1 hour ergometer cycling on 65% of Vo2max, and then the same as arm 1.
Intervention: Exercise (Other)
Fasting
3 days of fasting and then the same as arm 1
Intervention: Fasting (Other)
Control
control: muscle and fat biopsies and basal aminoacid and glucose turnover
Outcomes
Primary Outcomes
Growth hormone and insulin signal pathways
Time Frame: 30 min
Secondary Outcomes
- aminoacid and glucose turnover(4 hours)