Growth Hormone During Fasting. Signaltransduktion in Muscle and Adipose Tissue, Consequence of Growth Hormone Receptor Antagonist, Quantification of Intrahepatic Lipid Content Based on MR Scanning
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- insulin sensitivity
研究概览
简要总结
The purpose of this study is to examine the effects of growth hormone during fasting in healthy lean men.
详细描述
During fasting the human body is known to metabolize relatively large amounts of fat, in the expense of proteins and glucose. Partly this shift in metabolism is caused by increasing GH secretion, but exactly how growth hormone exerts these effects remains to be further investigated.
10 healthy lean young men are studied at 4 different occasions in a randomized single-blinded cross-over study. 1: after 12 hours of fasting + GH bolus, 2: after 36 hours of fasting + GH bolus, 3: after 36 hours + saline, 4: after 36 hours of fasting + Somavert.
Aim:
- to study the signal transduction in muscle and fat tissue
- to study the metabolism during fasting
- to study the intrahepatic fat content using magnetic resonance techniques
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 40 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •age 20 - 40 years of age
- •BMI 20 -25
排除标准
- •uses any medication
- •drinks more than 21 units of alcohol per
- •is claustrophobic
- •carries any magnetic devices
研究组 & 干预措施
1
12 hours of fasting and a GH bolus
干预措施: Somatropin and pegvisomant (Drug)
2
36 hours of fasting and a GH bolus
干预措施: Somatropin and pegvisomant (Drug)
3
36 hours of fasting and Pegvisomant
干预措施: Somatropin and pegvisomant (Drug)
4
36 hours of fasting and NaCl injection
干预措施: Somatropin and pegvisomant (Drug)
结局指标
主要结局
insulin sensitivity
时间窗: 36 hours of fasting
changes in intrahepatic lipid content
时间窗: 12 and 36 hours of fasting. respectively
changes in respiratory quotient
时间窗: 36 hours of fasting
changes in intracellular signaling during fasting
时间窗: 36 hours
metabolism
时间窗: 36 hours of fasting
forearm metabolism
时间窗: 36 hours of fasting
次要结局
- Changes in grelin(36 hours of fasting)
- changes in FFA(36 hours of fasting)
- changes in leptin, adiponektin, cortisol, catecholamine, glucagon, carbamide, palmitate(36 hours of fasting)