Kinetics of immune responses and characteristics of antigen-specific B cells after Bordetella pertussis vaccinatio
- Conditions
- PertussisWhooping cough10004018
- Registration Number
- NL-OMON52999
- Lead Sponsor
- eids Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 20
Healthy competent adults, who have received recommended vaccinations according
to the vaccination policy in the Netherlands
Subjects who meet any of the following criteria will be excluded from
participation in this study:
* has an auto-immune disease
* has an immune deficiency
* has a bleeding disorder
* underwent splenectomy
* receives immunosuppressive medication
* receives medication which influences blood clotting
* underwent active (clinically manifested) Bordetella pertussis infection at
any time point in life or had contact with such a person
* received Bp vaccination in the past 10 years
* has Hb level below 8.5 mmol/L (male) or below 7.5 mmol/L (female)
* is pregnant or breast feeding
* had an allergic response to vaccination in the past
* received any vaccination within the 14 days prior to the study
* donated blood or plasma within the month prior to the study, or is planning
to donate within the first month of the study
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Detailed analysis of innate and adaptive immune cells and their kinetics in<br /><br>time will be performed by the means of flow cytometry. Flow cytometry-based<br /><br>approach will be developed to identify antigen specific memory B cells and<br /><br>plasma cells prior to analysis of their immunoglobulin receptors by the means<br /><br>of high throughput sequencing. Study will be finished while all donors are<br /><br>recruited.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Not applicable</p><br>