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Effects of Lifestyle Intervention in the Biological Aging in Prediabetes

Not Applicable
Not yet recruiting
Conditions
PreDiabetes
Marker; Structural
Interventions
Other: Providing nutritional counselling
Other: counseling on physical activity
Registration Number
NCT06440681
Lead Sponsor
King Saud University
Brief Summary

Lifestyle modifications, including dietary changes and increased physical activity, play a crucial role in controlling complications to reduce or eliminate them. As well as controlling disturbances in biochemical markers such as DNAm PhenoAge, and signs of aging such as circulating sclerostin (SOST) and growth differentiation factor 15 (GDF15). This study seeks to address this dilemma by focusing on specific groups of elderly Saudis with prediabetes . Lifestyle changes in elderly Saudis with prediabetes show promise in reducing or eliminating complications. The potential insights derived from this research extend beyond academia, offering tangible benefits for clinical practice and public health

The proposed study will be implemented to achieve the following objective:

1. To determine changes in DNAm PhenoAge of elderly Saudis with prediabetes who underwent a 6-month lifestyle modification program.

2. To determine changes in other candidate senescence markers such as circulating sclerostin (SOST) and growth differentiation factor 15 (GDF15) of elderly Saudis with prediabetes who underwent a 6-month lifestyle modification program.

Detailed Description

Participants will be received at all healthcare centers in Arar, Kingdom of Saudi Arabia. They will be informed about the lifestyle modification intervention programme. The interview will take place in a designated and prepared place.

If approved, informed consent is signed. The procedures begin with filling out questionnaires and basic assessments include anthropometric, blood pressure and blood pressure measurements. The anthropometric measurements will be performed by well-trained medical staff. Height (m) and weight (kg) will be measured in light clothing without shoes. Waist, hip, circumference (cm) will be measured using a standard tape measure. BMI will be calculated by dividing weight in kilograms by the square of height in metres. Your blood pressure will be measured at each visit after adequate rest.

Dietary intake will be assessed through a validated food frequency questionnaire. Assessments will be repeated twice (at baseline and 6 months) in both groups. Fasting blood samples will then be collected using a sterile blood collection device to perform blood tests for biological age using self-analyzers in the internal laboratory of the participating primary health care centers. Tests will include complete blood count, serum albumin, creatinine, fasting glucose, C-reactive protein, alkaline phosphatase and other markers of aging such as SOST and DGF-15. Through these tests, the DNAm PhenoAge will be calculated through an online platform (https://thrivous.com/pages/biological-age Calculator).

In light of the results, participants will be included or excluded, and participants included in the program will continue with us through follow-up with nutritional awareness. 500 participants will be involved and all participants will be advised to:

Weight loss (5% of basic weight), exercise (4 hours/week), moderate fat consumption, and a diet rich in fiber.

5- Participants will receive individual, detailed and ongoing nutritional counseling through the study dietitian every two weeks, and they will also be encouraged to participate in physical activities. Table The detailed intervention for both groups is shown below. The measurements and analyzes will be repeated a few months after the beginning, and based on this, the Lifestyle Modification Group (LMP) will be determined: those who agreed and followed the previous instructions and advice.

The control group (CG): They are those who did not receive health education or did not follow previous instructions or advice.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
500
Inclusion Criteria
  1. Clinical diagnosis of pre-diabetes for all Saudis aged 30 years and above, males and females
  2. They must be able to exercise and be able to follow a diet

Exclusion Criteria

  1. Insulin-dependent diabetes and non-diabetic patients
  2. People with diseases that prevent them from participating and doing exercises
Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Lifestyle Modification Group (LMP)Providing nutritional counsellingThey are the people who will follow the lifestyle modification program, which is: Dietary counseling and monitoring: reduce fat intake (30% of total energy and saturated fat 10% of total energy) and increase fiber intake (15 g/1000 kcal) in the diet Physical activity: moderate exercise (150 minutes/week)
Lifestyle Modification Group (LMP)counseling on physical activityThey are the people who will follow the lifestyle modification program, which is: Dietary counseling and monitoring: reduce fat intake (30% of total energy and saturated fat 10% of total energy) and increase fiber intake (15 g/1000 kcal) in the diet Physical activity: moderate exercise (150 minutes/week)
Control group (CG)Providing nutritional counsellingThey are people who will not follow the lifestyle modification program or they did not know about this program
Control group (CG)counseling on physical activityThey are people who will not follow the lifestyle modification program or they did not know about this program
Primary Outcome Measures
NameTimeMethod
Change in the rate of measurements of Age marker Creatinine post intervention6 months

Change in Creatinine (umol/L)

Change in the rate of measurements of Age marker C-Reactive Protein post intervention6 months

Change in C-Reactive Protein (mg/dL)

Change in the rate of measurements of Age marker Lymphocyte post intervention6 months

Change in Lymphocyte (%)

Change in the rate of measurements of Age marker Albumin post intervention6 months

Change in Albumin (g/L)

Change in the rate of measurements of Age marker Alkaline Phosphatase post intervention6 months

Change in Alkaline Phosphatase (U/L)

Change in the rate of measurements of Age marker White Blood Cell Count (WBC) post intervention6 months

Change in White Blood Cell Count (WBC) (10\^3/uL)

Change in the rate of measurements of Age marker Mean Cell Volume (MCV) post intervention6 months

Change in Mean Cell Volume (MCV) (fL)

Change in the rate of measurements of Age marker Red Cell Distribution Width (RDW) post intervention6 months

Change in Red Cell Distribution Width (RDW) (%)

Change in the rate of measurements of Age marker fasting glucose post intervention6 months

Change in fasting glucose (mmol/L)

Secondary Outcome Measures
NameTimeMethod
Change in the rate of measurements of Age marker SOST post intervention6 months

Change in SOST(pmol/L)

Change in the rate of measurements of Age marker GDF15 post intervention6 months

Change in GDF15 (10\^3 pg/mL)

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