Determination of Gene Profiling in Semen Donors With Pregnancy Versus no Donor Gestation, Obtained in Assisted Reproduction Treatments
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 发起方
- 入组人数
- 14
- 试验地点
- 1
- 主要终点
- Detecting gene expression differences in RNA content messengers or gene expression profile between the semen samples versus those who get pregnant when you do not.
研究概览
简要总结
Microarray experiments to assess the expression of thousands of genes providing vast amount of information.
Because the molecular requirements in the different processes related to playback,on the hypothesis that there may be more apt semen samples for technical or other assuming molecular differences in RNA content messengers or gene expression profile between the They getting semen samples in gestation versus those who do not.
In an attempt to give a functional data obtained view, it is to find out how many of these genes in donors who have obtained gestation, they are differentially expressed in playback related processes against those donors who did not get pregnant.
详细描述
MRNA assembly is characterized by using microarrays on semen samples from donors included in the donation program of the clinic.
This process is performed in donors who have generated at least 3 pregnancies compared those samples, however, were not a function of gestation assisted reproduction technique used.
Consequently, differential expression of genes are identified in assisted reproduction treatment cycles from IAD, IVF or ICSI.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •IAD group Younger than 37 years, natural cycles and stimulated apparently no problem of infertility as follows:
- •Polycystic ovary syndrome.
- •Endometriosis.
- •ovarian failure.
- •Lack of tubal patency (Both fallopian tubes must submit permeability).
- •altered hormonal profiles (High levels of FSH, LH, testosterone and androstenedione).
- •Anovulation.
- •Uterine malformations (uterine septum, bicorne, didelphic and unicorn.)
- •Ovarian cysts or tumors.
- •Pelvic inflammatory disease.
- •Obesity (Women with a BMI> 30).
- •Thyroid Disease.
- •Diabetes mellitus.
- •renal or adrenal disease.
- •toxic habits (smoking, alcohol or drug abuse).
- •IVF group Couples who undergo IVF egg donation invoking the program. Therefore, they try to cycles in which two gametes were donors. All egg donors should be fertility proven, it is that must have been able to take shape within the program of egg donation or naturally. The recipient women (patients) should not present
- •Endometriosis.
- •Uterine malformations (uterine septum, bicorne, didelphic and unicorn.)
- •previous implantation failures.
- •cysts, polyps or uterine tumors.
- •Pelvic inflammatory disease.
- •Obesity (Women with a BMI> 30)
- •Thyroid Disease.
- •Diabetes mellitus. Renal or adrenal disease,
- •toxic habits (smoking, alcohol or drug abuse)
- •ICSI group Couples who undergo ICSI invoking the egg donation program. In addition, all donors oocytes must be of proven fertility, this being that should have been able to take shape within the egg donation program or naturally. The recipient women (patients) should not present
- •Endometriosis.
- •Uterine malformations (uterine septum, bicorne, didelphic and unicorn.)
- •previous implantation failures.
- •cysts, polyps or uterine tumors.
- •Pelvic inflammatory disease.
- •Obesity (Women with a BMI> 30)
- •Thyroid Disease.
- •Diabetes mellitus. Renal or adrenal disease,
- •toxic habits (smoking, alcohol or drug abuse)
排除标准
- 未提供
结局指标
主要结局
Detecting gene expression differences in RNA content messengers or gene expression profile between the semen samples versus those who get pregnant when you do not.
时间窗: 15 months
mRNA whole is characterized by using microarrays, in semen samples from donors included in the donation program of the clinic. By using bioinformatics tools Gene Ontology search listings of genes related to reproduction for the interpretation and identification of those who are involved in the different processes will be performed
次要结局
未报告次要终点