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99mTc-rhAnnexin V-128: a First In Man Study in Healthy Volunteers

Phase 1
Completed
Conditions
Healthy Adult Volunteers
Interventions
Drug: Kit for the Preparation of 99mTc-rhAnnexin V-128
Registration Number
NCT02182609
Lead Sponsor
Advanced Accelerator Applications
Brief Summary

The objectives of this study are:

* To determine the safety and tolerability of a single dose of 99mTc-rhAnnexin V-128 administered as an intravenous bolus over 10-20 seconds.

* To determine the biodistribution, pharmacokinetics and radiation dosimetry of 99mTc-rhAnnexin V-128 in normal healthy adult volunteers.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
14
Inclusion Criteria
  1. Age between 18 and 65 years
  2. No significant medical history
  3. Normal physical examination
  4. No clinically significant abnormalities in baseline laboratory values
  5. No clinically significant abnormalities on 12 lead electrocardiogram
  6. Female subjects must be post-menopausal, surgically sterilized or have negative urine beta human chorionic gonadotropin pregnancy test at initial screening and pre-injection on dosing day
  7. Written informed consent signed
Exclusion Criteria
  1. Pregnancy or lactation
  2. Know hypersensitivity to the investigational drug or any of its components
  3. Current enrolment in another investigational study
  4. Unwillingness to provide or continue informed consent

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
99mTc-rhAnnexin V-128Kit for the Preparation of 99mTc-rhAnnexin V-128-
Primary Outcome Measures
NameTimeMethod
Vital signsAt each study visit

Descriptive statistics of the vital signs (pulse rate and blood pressure) observed values as well as for the changes from baseline value will be created. Frequency tabulations with values within, below or above the normal ranges will be made.

Electrocardiographyscreening and immediatly, 24hrs, 72 hrs, 7 days and 30 days after injection

ECG parameters will include heart rate (HR), RR interval, PR interval, QRS width and QT interval.

ECG results will be evaluated by means of descriptive statistics and frequency tabulations.

Laboratory assessmentsbaseline and 24 hrs, 72 hrs and 30 days after injection

Laboratory data (Hematology, coagulation, biochemistry, and urinalysis) will be analysed with respect to the normal ranges of values provided by the local laboratory and with respect to pre-defined levels of change in these values.

Abnormal laboratory test results will be tabulated.

Adverse eventsWhole study period

Type and incidence of AEs, as well as severity and relatedness to the study medication will be tabulated. Special attention will be given to those subjects who prematurely discontinue the study or the study medication due to an AE, or who experience a severe AE or an SAE.

Secondary Outcome Measures
NameTimeMethod
Blood sample countingbaseline and 0, 5,10, 15, 30, 60, 90 min, 3 h, 6 h and 24 hours post-injection

Radioactivity of the whole blood and serum samples will be counted in a gamma counter to assess blood dosimetry and pharmacokinetic.

Amount of 99mTc excreted in faecesBaseline and within 24h post injection
Anti-rhAnnexin V-128 IgG and IgM antibodiesBaseline and 14 and 30 days post-injection
rhAnnexin V-128 serum concentration0, 5, 10, 15, 30, 60, 90, minutes and 3 h, 6h and 24 h post-injection

In addition to dosimetry, pharmacokinetic will be assessed based on rhAnnexin V-128 serum concentration

Whole-body SPECT imaging30min, 90min, 3hrs, 6hrs and 24 hours post-injection

The quantitative in vivo biodistribution (whole body dosimetry) of the 99mTc-rhAnnexin V-128 will be determined through whole-body SPECT imaging.

Amount of 99mTc excreted in urineOne sample at baseline and urine collection within 24 hours post-injection

Trial Locations

Locations (1)

The University of Ottawa Heart Institute

🇨🇦

Ottawa, Ontario, Canada

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