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Humoral and Cell Mediated Immunity and Safety of MF59C.1-adjuvanted Subunit Influenza Vaccine or a Conventional Subunit Influenza Vaccine in Previously Unvaccinated Healthy Subjects

Phase 2
Completed
Conditions
Seasonal Influenza
Human Influenza
Influenza
Influenza Due to Unspecified Influenza Virus
Interventions
Biological: MF59C.1-adjuvanted subunit influenza vaccine
Biological: Sub unit, Inactivated, Influenza vaccine
Registration Number
NCT01342796
Lead Sponsor
Novartis Vaccines
Brief Summary

This study aims to evaluate the immunogenicity, by means of cell mediated immunity (CMI) and hemagglutination inhibition (HI) assay, and also the safety of a MF59C.1-adjuvanted subunit influenza vaccine compared with a conventional subunit vaccine in previously unvaccinated children aged 6 to \<36 months.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
84
Inclusion Criteria
  • Male and female previously unvaccinated healthy children aged 6 to <36 months.
Exclusion Criteria
  • Any known or suspected impairment of the immune system, any serious disease.
  • Any subjects receiving licensed vaccines within 2 weeks (for inactivated vaccines) or 4 weeks (for live vaccines) prior to enrollment in this study.
  • Individuals who have had influenza vaccine or documented suspected influenza disease prior to day 1.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Arm 1MF59C.1-adjuvanted subunit influenza vaccine-
Arm 2Sub unit, Inactivated, Influenza vaccine-
Primary Outcome Measures
NameTimeMethod
Number of Subjects Reporting Unsolicited Adverse Events After Receiving Two Doses of aTIV and TIVDay 1 to Day 50 post vaccination

The number of subjects reporting unsolicited AEs between Day 1 and the study termination i.e., Day 50, after receiving two doses of aTIV and TIV. Data are reported based on the Safety Set.

Cell Mediated Immune (CMI) Responses After In-vitro Restimulation Of Peripheral Blood Mononuclear Cells (PBMC) Following Vaccination.Day 1, Day 50

The CMI responses were determined by intracellular staining/Fluorescence-Activated Cell Sorting(ICS/FACS) after in-vitro restimulation of PBMC with vaccine antigens on day 0 and day 50.

Secondary Outcome Measures
NameTimeMethod
Percentage of Subjects Achieving Seroconversion or Significant Increase in HI TiterDay 50

To evaluate the immune responses by seroconversion of aTIV or TIV according to CHMP criteria for seasonal influenza vaccines as determined by hemagglutination inhibition (HI) test on plasma from previously unvaccinated healthy children aged 6 to \< 36 months for all three strains.

Criteron: The proportion of subjects achieving seroconversion or significant increase in HI titer should be \> 40%.

Geometric Mean Ratios (GMR)Day 50/Day 1

To evaluate the immune responses by mean geometric increase (GMR) of aTIV or TIV according to CHMP criteria for seasonal influenza vaccines as determined by hemagglutination inhibition (HI) test on plasma from previously unvaccinated healthy children aged 6 to \< 36 months for all three strains.

Criterion: Mean geometric increase (GMR) should be \> 2.5.

Percentage of Subjects With HI Titers >1:40Day 50

To evaluate the immune responses by proportion of subjects with HI titers \>1:40 of aTIV or TIV according to CHMP criteria for seasonal influenza vaccines as determined by hemagglutination inhibition (HI) test on plasma from previously unvaccinated healthy children aged 6 to \< 36 months for all three strains.

Criterion: The proportion of subjects with HI titers \>1:40 should be \> 70%

Trial Locations

Locations (2)

Site 21: GZA campus Sint Vincentius

🇧🇪

Antwerpen, Belgium

Site 22: Kinderartsenpraktijk

🇧🇪

Hasselt, Belgium

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