Evaluating intracanal medicaments to reduce microbial count in root canal and inflammation reduction in tooth infection - Randomized controlled trial

Not yet recruiting

Conditions: Necrosis of pulp,

Registration Number: CTRI/2023/03/050533

Lead Sponsor: Subiksha Kannan

Brief Summary: INTRODUCTION:

One of the primary goals of root canal treatment is to eliminate or reduce the levels of microorganisms present in root canals below a threshold compatible with the healing of periapical tissues.â€˜Programmed resolutionâ€™ at the tissue level involves the participation of resolvins, a type of immuno resolvents, which actively drive the resolution of inflammation.

Embracing the concept of â€˜natural resolution of inflammationâ€™ and the aim of healing through tissue regeneration, an ideal intracanal medication should possess numerous desirable properties, such as antimicrobial activity, biocompatibility, and also must be capable of promoting endogenous production of lipid mediators that actively drive the resolution of inflammation.

AIM:

The aim of the present study is to clinically investigate the antimicrobial efficacy of chitosan, N- acetyl cysteine and calcium hydroxide as intracanal medicaments and their influence on level of inflammatory mediator Resolvin E1 in apical periodontitis.

JUSTIFICATION: Only one RCT evaluating the influence of intracanal medicaments on the levels of resolvins in apical periodontitis and one RCT evaluating antimicrobial efficacy of chitosan.

METHODOLOGY:

Root canal procedure will be performed by single Post-Graduate student under strict aseptic condition with local anesthesia (2% lidocaine & epinephrine at 1:200000) and rubber dam isolation. After complete instrumentation and irrigation, the teeth will be randomly divided into three groups according to the intracanal medicaments to be used.

GROUPS:

Group I : Chitosan (n=20)

Group II: N - Acetyl cysteine (n=20)

Group III: Calcium hydroxide (n=20)

Samples for microbiological analysis and resolvin level evaluation will be done before and after intracanal medicament placement.

Detailed Description: Not available

Recruitment & Eligibility

Status: Not Yet Recruiting

Sex: All

Target Recruitment: 60

Inclusion Criteria

1.Single-rooted 2.Pulp necrosis and apical periodontitis 3.
Presence of one root canal.

Exclusion Criteria

1.Patients who used antibiotics or antifungal medications in the past three months.
2.Root canal treated teeth 3.Any systemic disease 4.Periodontal pockets deeper than 4 mm 5.Teeth that could not be adequately isolated with a rubber dam 6.Acute apical abscess.

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Reduction of microbial load	3 months

Secondary Outcome Measures

Name	Time	Method
Level of RvE1 in periapical tissues	3 months

Trial Locations

Locations (1): SCB Dental college and Hospital
🇮🇳
Cuttack, ORISSA, India
SCB Dental college and Hospital
🇮🇳Cuttack, ORISSA, India
Subiksha Kannan
Principal investigator
9677963550
subikshakannan12@gmail.com