The Effect of Desfluran On Global Dna Methylation

Completed

• Conditions: DNA Damage
• Interventions: Drug: Desflurane

• Registration Number: NCT05111717
• Lead Sponsor: Muğla Sıtkı Koçman University

Brief Summary

The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

Detailed Description

Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Study Timeline

• Study First Submitted: 2021-10-17
• Study First Submitted QC: 2021-10-26
• Study First Posted: 2021-11-08
• Study Start: 2021-11-17
• Primary Completion: 2021-12-25
• Study Completion: 2021-12-30
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-14
• Last Update Posted: 2023-03-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

1. Ability to understand and willingness to sign an informed consent form
2. Elective lumbar spinal surgery that will take at least 120 minutes,
3. American Society of Anesthesiologists (ASA) physical status I and II
4. Be between the ages of 18 and 65

Exclusion Criteria

1. Patients with ASA physical status III or IV disease
2. Smoking or alcohol consumption
3. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders
4. recently received radiation, chemotherapy
5. malignancy
6. blood transfusion in operation
7. use of antioxidant supplements
8. have received general anesthesia in the last three months
9. Patients with known occupational exposures (operating room personnel, chemical plant workers

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
group 1	Desflurane	Peripheral venous blood samples will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). DNA will be obtained from blood samples , and global DNA methylation analysis will be done.

Primary Outcome Measures

Name	Time	Method
Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells	5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia	The global DNA methylation level will be measured 5 minutes before and 120 minutes after anesthesia induction.

Trial Locations

Locations (1)

Mugla Sıtkı Kocman University Training and research Hospital; 🇹🇷 Mugla, Turkey