The Effect of Desfluran Administered in General Anesthesia On Global Dna Methylation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells
研究概览
简要总结
The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.
详细描述
Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Ability to understand and willingness to sign an informed consent form
- •Elective lumbar spinal surgery that will take at least 120 minutes,
- •American Society of Anesthesiologists (ASA) physical status I and II
- •Be between the ages of 18 and 65
排除标准
- •Patients with ASA physical status III or IV disease
- •Smoking or alcohol consumption
- •Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders
- •recently received radiation, chemotherapy
- •blood transfusion in operation
- •use of antioxidant supplements
- •have received general anesthesia in the last three months
- •Patients with known occupational exposures (operating room personnel, chemical plant workers
研究组 & 干预措施
group 1
Peripheral venous blood samples will collected at two time points as follows: before the premedication and the induction of anesthesia (T₁): 120 minutes after the beginning of anesthesia (T₂). DNA will be obtained from blood samples , and global DNA methylation analysis will be done.
干预措施: Desflurane (Drug)
结局指标
主要结局
Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells
时间窗: 5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia
The global DNA methylation level will be measured 5 minutes before and 120 minutes after anesthesia induction.
次要结局
未报告次要终点
研究者
Melike Korkmaz Toker
associate professor
Muğla Sıtkı Koçman University