Genotoxic Effects of Desflurane and Propofol Anesthesia in Patients Undergoing Lumbar Disc Surgery
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
研究概览
简要总结
The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.
详细描述
It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Sequential
- 主要目的
- Diagnostic
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Ability to understand and willingness to sign an informed consent form
- •Elective lumbar spinal surgery that will take at least 120 minutes,
- •American Society of Anesthesiologists (ASA) physical status I and II
排除标准
- •Patients with ASA physical status III or IV disease
- •Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)
- •Smoking or alcohol consumption
- •Recently received radiation, chemotherapy
- •Patients who will receive blood transfusion during the operation
- •Users of antioxidant supplements
- •Patients who have received general anesthesia in the last three months
- •Patients with known occupational exposures (operating room personnel, chemical plant worker
研究组 & 干预措施
Desflurane Group
Desflurane 1,0 minimum alveolar concentration will be administered during anesthesia maintenance
干预措施: Anesthetics, General Desflurane Maintenance (Drug)
Propofol Group
Propofol 6 mg//kg/hour will be administered during anesthesia maintenance
干预措施: Anesthetics, General Propofol Maintenance (Drug)
结局指标
主要结局
Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
时间窗: postoperative 5th day
The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.
Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
时间窗: postoperative 5th day
The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.
Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
时间窗: postoperative 5th day
The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.
次要结局
未报告次要终点
研究者
Melike Korkmaz Toker
Associate Professor
Muğla Sıtkı Koçman University