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Effect of Strength Training and Protein Ingestion in Old Versus Very-old

Not Applicable
Completed
Conditions
Sarcopenia
Muscle Loss
Interventions
Procedure: Light Intensity Training
Procedure: Heavy Resistance Training
Procedure: Protein Whey
Registration Number
NCT02105922
Lead Sponsor
Bispebjerg Hospital
Brief Summary

From 3 interventiongroups in the project with ClinicalTrials.gov ID NCT02034760, namely:

HRTW: Heavy Strength Training x3/week \& 20g whey protein twice daily. LITW: Light Intensity Training x3-5/week \& 20g whey protein twice daily. WHEY: 20g whey protein twice daily.

15 subjects from each group will be recruited and tested after 3 months of intervention. Tests will include muscle cross sectional area (MRi), muscle biopsies (fiber types, size, cell- and capillary count), functional- and strength measurements, plasma lipids, HbA1c. Tests are to be compared with Project ID NCT01997320.

Detailed Description

In- and exclusion criteria are listed in NCT02034760. Subjects will be randomized to interventions as described, and 15 subjects from each of the three groups will be tested after 12 weeks of intervention.

15 subjects are included in each group to account for an expected drop-out rate of 20%, and so 12 subjects are expected to complete each intervention. With this N an increase of around 11% in MRi-evaluated muscle size can be detected with a powerlevel of 80% and an alpha of 0.05 and use of reported SD.

Primary outcome is muscle size (MRi), and secondary outcomes are overall body composition (DXA), muscle strength, rate of force development, power, 30s chair stand, 400m gait speed, grip strength. Tertiary outcomes are habitual activitylevel (accelerometry), blood samples (HbA1c, cholesterols, creatinine), blood pressure, weight, BMI, wast- and hip circumference.

Results are to be compared with NCT01997320 and so a population of 65+ can be compared with a population of 83+. Both studies uses the same heavy strength training regimen.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria
  • Healthy, independently living
  • Age at least 65 years
Exclusion Criteria
  • Subjects dependent on help/nursing etc.
  • Chronic medical diseases: Diabetes mellitus, clinical knee or hip osteoarthritis, other types of arthritis or connective tissue disorders, active cancer, renal diseases, severe chronic obstructive pulmonary disease, cardiac arrhythmias or known decreased left ventricular ejection fraction, lactose or gluten intolerance, chronic inflammatory bowel diseases, non-treated hyper/hypothyroidism, dementia.
  • Surgical diseases: Bone, muscle, tendon or joint injuries compromising participation in exercise regimens.
  • Implanted magnetic devices incompatible with MRi-scanning.
  • Weekly alcohol consumption > 21 units (1 unit equals 4g of ethanol) for men and > 14 for women.
  • Medicine except acetylsalicylic acid, paracetamol, thyroid function hormones, statins in doses above 40mg/day or if combined with subjective myalgia, ACE-inhibitors, Angiotensin II blockers, beta-blockers, calciumantagonists, proton-pump inhibitors, thiazides, potassium-sparing diuretics and loop diuretics.
  • >1 hour of exercise weekly, except light activities such as stretching/gymnastics and bike-riding/walking as transportation.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Light Intensity TrainingLight Intensity TrainingHome-based Light Intensity Training of the lower extremities three-five times weekly in combination with two daily 20g whey protein and 10g carbohydrate supplementations for 3 months.
Heavy Resistance TrainingHeavy Resistance TrainingHeavy Resistance Training of the lower extremities three times weekly in combination with two daily 20g whey protein and 10g carbohydrate supplementations for 3 months.
Protein WheyProtein WheyTwo daily 20g whey protein and 10g carbohydrate supplementations for 3 months.
Primary Outcome Measures
NameTimeMethod
Change in muscle cross sectional areaBaseline, 3 months

MRi scans of quadriceps muscle cross sectional area. The statistical evaluation of the primary outcome measure is done by applying a two way ANOVA test comparing groups: 1) Heavy Resistance Training vs. Light Intensity Training vs. Protein Whey. The statistical evaluation will be performed first as an intension-to-treat analysis and subsequently as a per-protocol analysis.

Secondary Outcome Measures
NameTimeMethod
Change in leg extension muscle powerBaseline, 3 months

Unilateral leg extension power measured in the Powerrig device.

Change from baseline in muscle structure and signallingBaseline and 3 months

From muscle biopsies muscle fiber type distribution and size, capillary density, satellite cell count and activity, selected gene-expression targets and protein concentrations will be assessed.

Change in 30 s chair standBaseline, 3 months

Number of stand-ups from a chair in 30 seconds.

Change in whole body composition and bone mineral densityBaseline, 3 months

Body composition (fat mass, lean mass and bone mass) evaluated by whole-body dual energy x-ray absorptiometry (DXA)-scanning.

Bone mineral density at dominant collum femoris and vertebrae L2-L4 evaluated by DXA-scanning.

Change in quadriceps muscle isokinetic strengthBaseline, 3 months

Unilateral knee extension (concentric) strength measured in the KinCom device at an angular velocity of 60 degrees/sec.

Change in quadriceps muscle isometric strengthBaseline, 3 months

Isometric unilateral quadriceps force and rate of force development measured in the isokinetic dynamometer device (KinCom) during knee extension at the knee angle 70 deg (with 0 degrees being fully extended).

Trial Locations

Locations (1)

Bispbebjerg Hospital

🇩🇰

Copenhagen NV, Denmark

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