Study on the Effect of Oligosaccharide Intake on Human Gut Microbiota

Not Applicable

• Conditions: Healthy adults

• Registration Number: JPRN-UMIN000053179
• Lead Sponsor: Obihiro University of Agriculture and Veterinary Medicine

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2023-12-20
• Study Completion: 2024-03-31
• Last Update Posted: 27 May 2024

Recruitment & Eligibility

• Status: Complete: follow-up continuing
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

Not provided

Exclusion Criteria

Not applicable

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
In a crossover intervention trial with a one-week washout period, this study aims to compare the gut microbiota in stool samples after a 14-day intake of an oligosaccharides (3g/day) and sugar (3g/day) as control using 16S rRNA amplicon sequencing analysis. And comparing effect of will be evaluatated among each diet on gut microbiota.

Secondary Outcome Measures

Name	Time	Method
In a crossover intervention trial with a one-week washout period, the study involves the intake of the test food (3.0 g of oligosaccharides) and the control food (3.0 g of sucrose) for 14 days. After the consumption period, the gut microbiota in stool samples will be subjected to in vitro fecal culture for 0-24 hours, and the resulting organic acid levels will be evaluated using HPLC (High-Performance Liquid Chromatography). Additionally, the study will assess the levels of organic acid (HPLC) and IgA (enzyme-linked immunosorbent assay)in the stool.