The Effect of Oral vs. Non-oral Contraceptive Therapy on Bone Turnover Using 41Ca Methodology
试验速览
- 阶段
- 4 期
- 状态
- 进行中（未招募）
- 入组人数
- 6
- 试验地点
- 2
- 主要终点
- Changes in 41Ca:Ca ratio
研究概览
简要总结
This preclinical randomized crossover study will examine the effects of oral versus non-oral contraceptive therapy on bone turnover in young exercising women. In an effort to expose the route-dependent effects of oral versus non-oral contraceptive therapy on bone turnover, the investigators will examine 1) the effects of ethinyl estradiol on bone calcium balance using the 41Ca technology and 2) the underlying physiological mechanisms associated with the effects of oral versus non-oral contraceptive therapy on net bone calcium balance.
详细描述
This study is a preclinical, multi-site study (Penn State University and Purdue University) that will determine whether the negative effects of combined oral contraceptive (COC) therapy on bone turnover are dependent on the route of administration such that an attenuation of these effects is observed when a comparable dose of non-oral contraceptive therapy, such as vaginal contraceptive (CVR) therapy is also tested. Millions of women use COC therapy for birth control purposes or regulation of menstrual cycles. CVR therapy is a relatively new FDA-approved contraceptive alternative to COC. The purpose of the proposed project is to investigate whether oral ethinyl estradiol (EE) may negatively impair bone turnover when compared to vaginally-administered EE in young physically-active women.
A novel calcium tracer technology suitable to rapidly assess changes in net bone calcium balance in a small sample size will be applied to evaluate the effects of COC and CVR therapy on bone turnover. The radioisotope Calcium-41 (41Ca) demonstrates increased sensitivity when compared to dual x-ray absorptiometry (DXA) and increased specificity for bone mineralization and precision when compared to biochemical markers of bone turnover. This study will be the first study to examine how the route of estrogen administration affects bone turnover in young physically active women by assessing changes in net bone calcium balance with different forms of contraceptive therapy.
The overall purpose of this study is to explore the effects of oral versus vaginal contraceptive therapy on bone turnover in young exercising women using 41Ca technology. In an effort to expose the route-dependent effects of oral versus non-oral contraceptive therapy on bone turnover, the investigators will examine 1) the effects of ethinyl estradiol on bone calcium balance using the 41Ca technology and 2) the underlying physiological mechanisms associated with the effects of oral versus vaginal contraceptive therapy on net bone calcium balance.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 25 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Age 18-25 yrs
- •BMI 18-29 kg/m2
- •Non-smoking
- •Willing to refrain from using hormonal contraceptives for the equilibration + baseline period of the study (~150 days)
- •Availability to frequently visit the laboratory in the next 12 months
- •Not currently pregnant nor intending to become pregnant in the next 12 months
- •Not lactating
- •No apparent metabolic, endocrine, musculoskeletal, or severe psychiatric disease
- •Willing to maintain consistent exercise (aerobic, weight-bearing) and dietary habits during pre-intervention and intervention periods
- •Willing to refrain from supplements other than those provided by study staff in the next 12 months
- •Variable physical activity acceptable, but mode must be primarily weight bearing
- •At least 9 menses in the past 12 months
排除标准
- •Non-weight bearing exercise as primary mode of physical activity
- •Resistance exercise training (≥ 2 times 30 minutes per week of resistance exercise)
- •Complete seasonal change of impact mode of physical activity
- •Known or suspected metabolic or endocrine disease
- •Currently consuming large amounts of soy products
- •Current clinical eating disorder or other axis 1 psychiatric disorder or bipolar disorders
- •Oral or hormonal contraceptive use in the last 6 months
- •Hyperparathyroidism
- •Liver or renal disease
- •Evidence of malabsorption or skeletal disorder
- •Thyroid abnormalities (controlled hypothyroidism acceptable)
- •Chronic use of non-steroidal anti-inflammatory drugs (NSAIDS)
- •Taking medications known to have interactions with oral or vaginal contraceptive therapy
- •Other Exclusion Criteria proposed by the World Health Organization COC Contraindications (Grossman et al., 2011).
研究组 & 干预措施
Combined Oral Contraceptive (COC)
We are testing the effects of a COC (Apri (Reclipsen); 30 µg EE, 150 µg desogestrel). On the first day of the intervention, the participants randomized to COC will begin taking the pill. One pill will be ingested orally at the same time each day for days 1-49 of the intervention. Pills ingested during days 1-21 and during days 29-49 will be active tablets. Pills ingested on days 22-28 will be tablets without active ingredients. Each participant in the COC group will ingest a pill from the first pack each day for the first 28 days then begin the second pack. If a participant is unable to collect a 24-h urine sample on day 49, she will take active tablets from the third pill pack until she has collected the 24-h urine sample.
干预措施: Combined Oral Contraceptive (COC) (Drug)
Contraceptive Vaginal Ring (CVR)
We are testing the effects of the vaginal ring (Nuva Ring; 15 µg/d EE, 120 µg/d etonogestrel). When randomized to CVR, participants will insert the contraceptive ring into their vagina . The ring will be worn during weeks 1-3, and again during weeks 5-7. During week 4, no ring will be worn to allow for withdrawal bleeding. If a participant is unable to collect a 24-h urine sample on day 49, they will insert a third ring which will remain in the vagina until she has collected a 24-h urine sample.
干预措施: Contraceptive Vaginal Ring (CVR) (Drug)
结局指标
主要结局
Changes in 41Ca:Ca ratio
时间窗: 49 days of contraceptive therapy
Changes in the 41Ca:Ca ratio in 24-h urine samples measured every 10-14 days during and after oral and non-oral contraceptive therapy as determined by Accelerometer Mass Spectrometry
次要结局
- Changes in serum concentrations of markers of bone formation and bone resorption(49 days of contraceptive therapy)
- Changes in serum concentrations of insulin-like growth factor-1 (IGF-1), IGF-binding proteins (IGFBP-1, IGFBP-3), and acid labile subunit (ALS)(49 days of contraceptive therapy)
研究者
Mary Jane DeSouza
Professor of Kinesiology and Physiology
Penn State University