MedPath

Microcirculation and Inflammatory Markers in Patients With Resistant, Stages 1/2 Hypertension and Normal Blood Pressure

Completed
Conditions
High Blood Pressure
Registration Number
NCT02759276
Lead Sponsor
Daniel Alexandre Bottino
Brief Summary

Nailfold videocapillaroscopy (NVC), Venous occlusion Plethysmography (VOP) and inflammatory biomarkers have been used to study endothelial function in patients with resistant hypertension, stages 1 and 2 hypertension and normotensive. This was a cross-sectional study.

Detailed Description

Microcirculation determines peripheral resistance contributing to increased blood pressure. The nailfold videocapillaroscopy (NVC), Venous occlusion Plethysmography (VOP) and biomarkers can be used to evaluate the microcirculation in arterial hypertension (AH). The objective of this study is to analyze the behavior of serum inflammatory markers and study the microcirculation by nailfold videocapillaroscopy (NVC) and Venous Occlusion Plethysmography in patients with resistant hypertension (RH), with stages 1 and 2 hypertension (MMH ) and normotensive (CG). A cross-sectional study with 75 patients has been performed: 25 with resistant hypertension (RH), 25 with stages 1 and 2 hypertension (MMH) and 25 normotensive (CG). All underwent anamnesis, dosage serum markers and conducting NVC, VOP. Hypertensive patients without statin use and with controlled or uncontrolled blood pressure were also analyzed. For the statistical analysis were used: Kruskal -Wallis ANOVA, Dunn test and ANCOVA for correction of confusion factors such as age and BMI.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
75
Inclusion Criteria
  • Clinical diagnosis of controlled High Blood Pressure stages 1 and 2 (blood pressure levels from 140/90 mmHg to 179/109 mmHg) .
  • Resistant Hypertension (uncontrolled hypertension, with values ≥140/90 mmHg, using three or more antihypertensive drugs of different classes, including a diuretic, or controlled hypertension with four or more drugs).
  • Normal Blood Pressure.
Exclusion Criteria
  • Diabetes Mellitus, Types 1 or 2.
  • Heart Failure.
  • Myocardial infarction or stroke with less than three months of the event.
  • Chronic kidney disease.
  • Use of hormonal or non-hormonal anti-inflammatory drugs.
  • Recent trauma (less than three months).
  • Autoimmune diseases.
  • Infectious processes in activity.
  • Neoplasia.
  • Aspirin use (anti-inflammatory dose).
  • Obesity grade III (Body Mass Index>40 kg/m2).

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Blood Pressure (mmHg)1 day

Hemodynamic Variable

Secondary Outcome Measures
NameTimeMethod
Functional Capillary Density (FCD, number capillaries/mm2 )1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Apical Capillary Diameter (micrometer)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Afferent Capillary Diameter (micrometer)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Efferent Capillary Diameter (micrometer)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Maximal Forearm Blood Flow after sublingual nitroglycerine1 day

Vascular Reactivity Variables from Venous Occlusion Plethysmography

Intercellular Adhesion Molecule (ICAM, ng/dl)up to 2 weeks

Inflammatory Biomarker

Time to reach Maximum Red Blood Cell Velocity (s)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Maximal Forearm Blood Flow during reactive Hyperemia1 day

Vascular Reactivity Variables from Venous Occlusion Plethysmography

Endothelin (pg/dl)up to 2 weeks

Inflammatory Biomarker

Waist circumference (cm)1 day

Anthropometrical Variable

Hip circumference (cm)1 day

Anthropometrical Variable

Vascular Cell Adhesion Molecule (VCAM, ng/dl)up to 2 weeks

Inflammatory Biomarker

Adiponectin (ng/dl)up to 2 weeks

Inflammatory Biomarker

C Reactive Protein (CRP, mg/dl)up to 2 weeks

Inflammatory Biomarker

Red Blood Cell Velocity (mm/s)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Maximum Red Blood Cell Velocity (mm/s)1 day

Microcirculatory variable from Nailfold Videocapillaroscopy

Body Mass Index (Kg/m2)1 day

Anthropometrical Variable

Waist to Hip Ratio1 day

Anthropometrical Variable

© Copyright 2025. All Rights Reserved by MedPath