Examining the Relationship Between Acute Meal Intake and Inflammation in Children

Not Applicable

Completed

• Conditions: Obesity; Inflammation
• Interventions: Other: Eggs and Carbohydrates

• Registration Number: NCT03597542
• Lead Sponsor: University of Illinois at Urbana-Champaign

Brief Summary

Recent evidence has shown that obese and overweight children exhibit states of chronic inflammation. In obese adults, a high carbohydrate meal induces an inflammatory response; however, the effects of a high carbohydrate meal on biomarkers of inflammation has not previously been examined in children. The purpose of this research project is to characterize the inflammatory response to a high carbohydrate versus a low carbohydrate meal in healthy weight, overweight, and obese children (age 7-17 years). After completing informed consent/assent, a Dual-energy X-ray Absorptiometry (DXA) scan, and baseline blood draw on experimental day 1, children will then return to the lab two times to ingest either a high or low carbohydrate beverage followed by post-meal blood collection for a total of 3 blood draws. Biomarkers of inflammation will be analyzed by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Participants will be recruited through various studies through the Neurocognitive Kinesiology Lab/Body Composition and Nutritional Neuroscience Labs as well as through the local Champaign-Urbana community. Data from this project will provide a better understanding of the inflammatory response to different meals in healthy weight, overweight, and obese children.

Detailed Description

Not available

Study Timeline

• Study Start: 2017-08-29
• Primary Completion: 2018-02-02
• Study Completion: 2018-02-02
• Status Verified: 2018-07
• Study First Submitted: 2018-07-13
• Study First Submitted QC: 2018-07-13
• Study First Posted: 2018-07-24
• Last Update Submitted: 2018-07-28
• Last Update Posted: 2018-07-31

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 36

Inclusion Criteria

• Between the ages of 7-17

Exclusion Criteria

• Younger than 7 years and older than 17 years
• Presence of allergies to eggs and/or sugar substitutes
• Presence of heart or respiratory disease
• Presence of uncontrolled hypertension, diabetes
• Use of anti-inflammatory medications
• Presence of cancer or metabolic disease

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Maltodextrin	Eggs and Carbohydrates	Participants will consume 56g of maltodextrin dissolved in 500mL of water.
Egg	Eggs and Carbohydrates	Participants will consume 36g of spray-dried egg powder (equivalent to 3 eggs) dissolved in 500mL of water.

Primary Outcome Measures

Name	Time	Method
Effects of egg or maltodextrin on inflammatory cytokines (Tumor necrosis factor-alpha, C-reactive protein, and Interleukin-6)	3 weeks.	Will be used by determining any changes in inflammatory cytokines via enzyme-linked immunosorbent assay (ELISA) kits.

Secondary Outcome Measures

Name	Time	Method
Effects of egg or maltodextrin on inflammatory monocyte cells with and without expression of Toll-like receptor 4 and C-C Chemokine receptor-2.	3 weeks.	Will be used by determining the cells per microliter of lysed whole blood.

Trial Locations

Locations (1)

University of Illinois at Urbana-Champaign; 🇺🇸 Urbana, Illinois, United States