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Preclinical Project on the Traitment of Acute Lymphoblastique Leukemia With NVP-BEP800, an Inhibitor of the Heat Shock Protein HSP90

Conditions
Acute T Lymphoblastic Leukemia (T-ALL)
Acute B Lymphoblastic Leukemia (B-ALL)
Interventions
Drug: injection of BEP 800
Other: Control
Registration Number
NCT04437420
Lead Sponsor
Centre Hospitalier Universitaire Dijon
Brief Summary

NVP-BEP800, a new HSP90 inhibitor, has particularly interesting therapeutic potential and represents hope in cancer pathologies. While it is currently being tested for solid cancers, no preclinical study has yet demonstrated its effectiveness in acute lymphoblastique leukemia (ALL). The investigators wish to study the effects of NVP-BEP800 on two different types of ALL (T and B-ALL).

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
44
Inclusion Criteria
  • Patient suffering from acute lymphoblastic leukaemia whose biological samples are kept at the Biology Technical Platform.
Exclusion Criteria
  • Patient over 80 years of age

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
T-ALLinjection of BEP 800-
T-ALLControl-
B-ALLinjection of BEP 800-
B-ALLControl-
Primary Outcome Measures
NameTimeMethod
Viability on primary ALL samples2 years

The effect of BEP-800 after 48 hours of treatment on T-ALL and B-ALL will be evaluated by counting viable cells by flow cytometry with a viability marker and specific antibodies to detect leukemia cells.

Secondary Outcome Measures
NameTimeMethod
Effect on SRC phosphorylation2 years

The effect of BEP-800 on T-ALL and B-ALL will be evaluated by flow cytometry by measuring HSP90 and SRC (phosphorylated), by intracellular labeling after permeabilization of the cells, 18 hours after treatment with BEP-800.

Trial Locations

Locations (1)

Chu Dijon Bourgogne

🇫🇷

Dijon, France

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