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Yttrium Y 90 Anti-CD19 Antibody BU-12 in Patients With Advanced Relapsed or Refractory Acute Lymphoblastic Leukemia or Chronic Lymphocytic Leukemia

Phase 1
Terminated
Conditions
Leukemia
Interventions
Radiation: yttrium Y 90 anti-CD19 monoclonal antibody BU12
Radiation: 111In-BU-12
Registration Number
NCT00643240
Lead Sponsor
Masonic Cancer Center, University of Minnesota
Brief Summary

RATIONALE: Radiolabeled monoclonal antibodies can find cancer cells and carry cancer-killing substances to them without harming normal cells. This may be effective treatment for leukemia.

PURPOSE: This phase I trial is studying the best dose of yttrium Y 90-labeled monoclonal antibody BU-12 in treating patients with advanced relapsed or refractory acute lymphoblastic leukemia or chronic lymphocytic leukemia.

Detailed Description

OBJECTIVES:

Primary

* To determine the biodistribution of indium-111 BU-12 in patients with refractory CD19+ leukemia.

Secondary

* To determine the maximum tolerated dose of yttrium Y 90 anti-CD19 antibody BU-12

* Determine the human anti-mouse antibody (HAMA) response.

* To define, preliminarily, the antitumor activity of yttrium Y 90 anti-CD19 antibody BU-12.

OUTLINE: Patients receive yttrium Y 90 anti-CD19 antibody BU-12/indium-111 BU-12 IV over 60 minutes on day 0 and undergo whole-body imaging on days 0, 1, 3, 4, and 7. Patients also undergo blood collection and bone marrow biopsy periodically for dosimetry calculations and pharmacokinetics.

After completion of study treatment, patients are followed periodically for 2 years.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
1
Inclusion Criteria
  • Histologically confirmed CD19-positive (> 25% by flow cytometry evaluation of bone marrow blasts) disease of 1 of the following types:

    • Primary refractory or relapsed acute lymphoblastic leukemia (ALL) defined as persistent disease following a minimum of two different standard effective chemotherapy induction attempts at time of diagnosis or at relapse
    • Chronic Lymphocytic leukemia (CLL) following blast crisis (≥15% bone marrow blasts following a minimum of one standard effective chemotherapy induction attempt)
  • Human anti-mouse antibody (HAMA) must be negative

  • Patients who have relapsed ≥ 60 days following an autologous or allogeneic transplant are eligible if all other eligibility criteria are met

  • No active central nervous system (CNS) disease

  • ECOG performance status (PS) 0-2 or Karnofsky PS 60-100%

  • Life expectancy > 8 weeks

  • Total bilirubin ≤ 2.5 times upper limit of normal (ULN)

  • AST and ALT ≤ 2.5 times ULN

  • Creatinine normal OR creatinine clearance ≥ 60 mL/min

  • LVEF ≥ 45% by MUGA/ECHO

  • Oxygen saturation on room air > 92% and no oxygen requirement

  • Not pregnant or nursing

  • Negative pregnancy test

  • Fertile patients mus use effective contraception

Exclusion criteria:

  • History of allergic reactions attributed to compounds of similar chemical or biologic composition to of yttrium Y 90 anti-CD19 antibody BU-12 or other agents used in study

  • Uncontrolled illness including, but not limited to, any of the following:

    • Ongoing or active infection
    • Symptomatic congestive heart failure
    • Unstable angina pectoris
    • Cardiac arrhythmia
    • Psychiatric illness/social situations that would limit compliance with study requirements
  • HIV-positive

  • Active graft-vs-host disease

  • Less than 4 weeks since prior agents and recovered

  • Less than 7 days since prior therapy with any biologic agent, defined as a growth factor or cytokine

  • Less than 3 months since prior antibody or biologic anticancer therapy (e.g., alemtuzumab or epratuzumab)

  • Other concurrent investigational agents

  • Patients with peripheral blasts > 5,000/uL may receive concurrent hydroxyurea

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
111 In-BU-12yttrium Y 90 anti-CD19 monoclonal antibody BU12111In-BU-12 is the 111Indium-labeled murine monoclonal antibody used for imaging and dosimetry.
111 In-BU-12111In-BU-12111In-BU-12 is the 111Indium-labeled murine monoclonal antibody used for imaging and dosimetry.
Primary Outcome Measures
NameTimeMethod
Biodistribution of indium-111 BU-12Immediately post infusion, 4-6 hours after infusion and Days 1, 3, 4 and 7 after infusion

Perform a whole body scan acquiring both anterior and posterior images at a speed of 10 cm/min (20 minute scan) using a medium energy collimator, a 256 x 1024 computer acquisition matrix and acquisition photo peak settings of 172 and 247 keV with 15% windows.

Secondary Outcome Measures
NameTimeMethod
Presence or absence of a human antibody to murine antibodybaseline, 28 and 60 days post therapy, and at 6 months post therapy

Presence or absence of a human antibody to murine antibody at baseline, 28 and 60 days post therapy, and at 6 months post therapy

Maximum tolerated dose of yttrium Y 90 anti-CD19 antibody BU-12Beginning Day 1 of treatment

DLT will be defined as bone marrow aplasia \> 6 weeks duration from the first treatment day; specifically, failure to recover peripheral ANC \> 500/μL and platelets \> 20,000/μL documented by bone marrow aplasia, not malignant infiltration - Any NCI CTCAE v 3.0 grade 3 non-hematologic toxicity except for allergic reactions to radiolabeled BU-12 will be dose limiting. If the BU-12 antibody is very allergenic, then ≥ grade 3 allergic reactions will be dose limiting.

Number of Patients by Clinical Responseday 28 and day 60

Patients evaluable for DLT will be assessed for response at day 28 and day 60 post therapy dose (event is whether or not the patient has a Complete Remission, Partial Remission, Stable Disease, Refractory Disease, Relapsed Disease). The proportion of patients by disease status will be reported.

Time to Clinical ResponseDay 28, 60, 6 Months

Time-to-event will be measured from date of therapy dose.

Trial Locations

Locations (1)

Masonic Cancer Center at University of Minnesota

🇺🇸

Minneapolis, Minnesota, United States

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