Influence of a 12-week supplementation with collagen peptides and resistance training on the intracellular signalling pathway of muscular protein biosynthesis as well as ultrastructural and functional adaptations in skeletal muscles

Not Applicable

Conditions: Effects of resistance training and collagen peptide intake on muscular adaptations in healthy volunteers

Registration Number: DRKS00027112

Lead Sponsor: Institut für Sport und Sportwissenschaft, Albert-Ludwigs-Universität Freiburg

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Complete

Sex: Male

Target Recruitment: 30

Inclusion Criteria

Male gender
- age between 18-29
- BMI 18.5 - 25
- Physically active:
- < 150 minutes/week of moderate-intensity exercise (e.g. 5 x 30 minutes/week) or < 75 minutes/higher-intensity exercise (running, cycling, playing sports).
- No regular participation in strength training during the last 12 months
- Stable weight and nutritional behaviour (mixed diet)
- Informed consent
- No contraindications to exercise according to ACSM guidelines
- Metabolically healthy

Exclusion Criteria

- Regular participation in strength and endurance training during the last 12 months.
- Malignant diseases in the past 5 years.
- Cardiac disease (e.g. chronic heart disease, arrhythmia, valvular disease, arthritis etc.). Cardiac disease (e.g. chronic heart disease, arrhythmia, heart valve disease, arthritis, etc.).
- Arterial hypertension (RRsyst > 200 mmHg and/or RRdiast > 105 mmHg) at rest.
- Presence of insulin-dependent diabetes mellitus (IDDM).
- Presence of a lipometabolic disorder.
- Regular intake of medication.

Study & Design

Study Type: interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Change in mTOR activation / phosphprylation

Secondary Outcome Measures

Name	Time	Method
All secondary outcomes will be investigated either in the acute study (single strength training session with supplementation) or in the long-term study (12-week):<br><br>Acute study:<br>- Genome sequencing/transcriptome sequencing-.<br>- Protein activation (AKT, AMPK, FoXo, MuRF-1, MAFbx, PRAS40, ERK1/2, LCB3, LBC I/II, Antrogin-1).<br><br>Long-term study:<br>- Histological examinations (muscle fibre distribution, muscle cell nucleus density, fibroblast density, satellite cell distribution)<br>- Protein concentrations (AKT, AMPK, FoXo, MuRF-1, MAFbx, PRAS40, ERK1/2, LCB3, LBC I/II, Antrogin-1)<br>- Muscle volume (MRI)<br>- Tendon volume (MRT)<br>- Muscle strength