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Effects of Coffee on Hepatic Steatosis Induced by a High Fructose Diet

Not Applicable
Completed
Conditions
Hepatic Steatosis
Interventions
Dietary Supplement: Ctl
Dietary Supplement: High fructose diet; no coffee
Dietary Supplement: fully torrefied, caffeine-free coffee
Dietary Supplement: partially torrefied, caffeine-free coffee
Dietary Supplement: Partially torrefied, caffeinated coffee
Registration Number
NCT00827450
Lead Sponsor
University of Lausanne
Brief Summary

This study will assess

* whether coffee consumption protects against fructose-induced hepatic steatosis in healthy humans

* whether the protective effect of coffee is dependent on it's antioxidant composition

Detailed Description

Epidemiological studies suggest that coffee consumption improves glucose homeostasis in insulin resistant subjects. An increase in intrahepatic lipids (hepatic steatosis) is highly prevalent in patients with the metabolic syndrome and may be used as a marker of altered hepatic lipid metabolism. Such an increased hepatic lipids content can be experimentally produced in healthy humans by a 6-day high fructose diet.

The purpose of this study is to evaluate whether coffee prevents hepatic lipid deposition in healthy male subjects fed a fructose-rich hypercaloric diet. Both caffeine and antioxidants (yet unspecified) may be involved.. To sort out the role of caffeine and antioxidants, we will test 3 different soluble coffee, ie fully torrefied decaffeinated coffee , partially torrefied decaffeinated coffee, and partially torrefied caffeinated coffee.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
13
Inclusion Criteria
  • BMI between 19 and 15 kg/m2
  • less than 30 min physical activity /day
  • habitual coffee consumption less than three cupy /day
  • consumption of caffeine-containing sodas less than 2 servings/day
  • non-smoker
Exclusion Criteria
  • consumption of alcohol more than 40g/day
  • presence of metallic foreign bodies
  • history of eye surgery
  • family history of diabetes mellitus
  • history of food intolerance
  • vegetarians

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
CtlCtlcontrol isocaloric diet; no coffee
HFHigh fructose diet; no coffeeHypercaloric. high fructose diet; no coffee
C1fully torrefied, caffeine-free coffeeHypercaloric, high fructose diet; caffeine-free, torrefied coffee
C2partially torrefied, caffeine-free coffeeHypercaloric, high fructose diet; caffeine-free, partially torrefied coffee
C3Partially torrefied, caffeinated coffeeHypercaloric, high fructose diet; caffeinated, partially torrefied coffee
Primary Outcome Measures
NameTimeMethod
intra-hepatocellular lipid (IHCL) concentrationwill be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
Secondary Outcome Measures
NameTimeMethod
fasting plasma triglycerideswill be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
fasting net lipid oxidationwill be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
fasting net carbohydrate oxidationwill be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
whole body ketone bodies turnover and oxidation (13C 3-hydroxybutyrate)will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
whole body glucose turnover (6,6 2H2 glucose)will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
whole body glycerol turnover (2H5 glycerol)will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement

Trial Locations

Locations (1)

Centre d'investigations cliniques "cardiomet"/ CHUV

🇨🇭

Lausanne, Switzerland

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