MedPath

The Underlying Mechanisms Regarding the Effect of Glucagon on the Kidneys Will be Investigated in Healthy Males.

Not Applicable
Recruiting
Conditions
Kidney Diseases
Registration Number
NCT06498063
Lead Sponsor
Ali Asmar
Brief Summary

The goal of this crossover study is to investigate to what extend glucagon affects the kidneys. The main questions it aims to answer are:

Does glucagon regulate kidney function through extraction in the kidney in addition to glomerular filtration? Does glucagon regulate kidney function by increasing renal plasma flow and glomerular filtration rate? Does glucagon regulate kidney function by increasing renal salt excretion?

Detailed Description

In patients with type 2 diabetes mellitus, plasma concentrations of glucagon are inappropriately high (hyperglucagonemia). Hyperglucagonemia has been speculated to contribute to the pathophysiology of diabetic kidney disease. Previously, glucagon has been assumed to cause glomerular hyperfiltration associated with urinary excretion of small proteins, a characteristic of early type 2 diabetic kidney injury. Further, glucagon has been shown to acutely increase urinary excretion of urea, sodium, and potassium, and patients with end-stage renal disease have elevated plasma levels of glucagon.

The purpose of this study is to clarify the underlying mechanisms behind the physiological effects of glucagon on kidney function and the kidney's ability to clear glucagon from the blood in healthy males. Specifically, the investigators aim to answer the following questions:

Does glucagon regulate kidney function through extraction in the kidney in addition to glomerular filtration? Does glucagon regulate kidney function by increasing renal plasma flow and glomerular filtration rate? Does glucagon regulate kidney function by increasing renal salt excretion?

The renal extraction of glucagon and the renal effects of glucagon will be investigated during a constant glucagon infusion in 10 healthy men aged 20-60 years. The study will be placebo-controlled. Each subject will participate in three independent and randomized trial days with a washout period of at least four weeks.

Recruitment & Eligibility

Status
RECRUITING
Sex
Male
Target Recruitment
10
Inclusion Criteria
  • Age: 20-60 years
  • Normal health ascertained through questioning and medical examination
  • Normal values for blood concentrations of fasting plasma glucose, fasting plasma total cholesterol, fasting triglycerides, HDL, LDL, creatinine, liver function, and electrolytes
  • Informed consent
Exclusion Criteria
  • Immunosuppressive treatment in the preceding 12 months
  • Alcohol abuse
  • Medical treatment with oral glucocorticoids, dipeptidyl peptidase-4 (DPP-4) inhibitors, or GLP-1 receptor agonists, which, in the opinion of the investigator, may interfere with glucose metabolism
  • Use of lithium
  • Medical treatment that affects insulin secretion or cardiovascular performance measures
  • Liver disease (ALT > 2x normal value)
  • Renal impairment (se-creatinine > 130 μM and/or albuminuria)

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Glucagon extractionAnalyzed from blood samples drawn at -30, 0, 20, 40, 60, 80, 100, 120, 140, 160 and 180 minutes

From blood samples, unit pmol/L

NatriuresisAnalyzed from urine samples at -60, 0, 60 and 120 minutes

From urine samples, unit mmol/L

Secondary Outcome Measures
NameTimeMethod
Glomerular filtration rateMeasured via Fick's principle during steady state using [99mTc]Tc-DTPA (diethylene-triamine-pentaacetate) as a tracer given as a constant infusion from -210 to 180 min.

Unit mL/min

DiuresisAnalyzed from urine samples at -60, 0, 60 and 120 minutes

from urine samples, unit mL/min

Renal Blood FlowMeasured via Fick's principle during steady state using [99mTc]Tc-DTPA (diethylene-triamine-pentaacetate) as a tracer given as a constant infusion from -210 to 180 min.

Unit mL/min

UreaAnalyzed from blood samples drawn at -30, 0, 20, 40, 60, 80, 100, 120, 140, 160 and 180 minutes

Unit mg/dL

Trial Locations

Locations (1)

Physiological laboratory, Bispebjerg Hospital, Research Unit, Clinical Physiology / Nuclear Medicine Department

🇩🇰

Copenhagen, Denmark

Physiological laboratory, Bispebjerg Hospital, Research Unit, Clinical Physiology / Nuclear Medicine Department
🇩🇰Copenhagen, Denmark
Anna Billeschou Bomholt, M.Sc.
Contact
800-555-5555
anna.billeschou@sund.ku.dk
Anna Billeschou Bomholt, PhD fellow
Sub Investigator

MedPath

Empowering clinical research with data-driven insights and AI-powered tools.

© 2025 MedPath, Inc. All rights reserved.