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The Influence of Football Training as a medicine_FIM_UTH

Not Applicable
Completed
Conditions
Physical Activity
Body Composition
Healthy
Soccer Training
Interventions
Behavioral: FIM-1
Behavioral: FIM-2
Behavioral: Control
Behavioral: FIM-3
Registration Number
NCT05273203
Lead Sponsor
University of Thessaly
Brief Summary

The purpose of this randomized controlled study will be to inestigate the relationship between recreational football training at various training volumes and the health responses of middle-aged participants at high risk for metabolic and / or cardiovascular disease.

Detailed Description

A controlled, randomized, four-group, repeated-measures clinical trial will be apllied. Males and females middle-aged volunteers (age 40-60 years) will participate in the present study. The participants will not suffer from any musculoskeletal injuries that will limit their ability to perform the exercise protocols.

The study will be consisted of the following stages:

1. In the first phase all participants will sign an informed consent form after they will be informed about all benefits and risks of this study and they will sign a recent historical of musculoskeletal injury or illness form.

2. Initial testing: body weight (BW) and height, BMI, electrocardiogram (ECG), RMR, daily physical activity (PA) and daily nutritional intake (participants will be instructed by a dietitian how to record a 7 days diet recalls).

3. A 4-week adaptive period: based on a dietary analysis, participants will be given a dietary plan (considering the RMR and total daily physical activity related energy expenditure), providing an isocaloric diet over the initial 4-week adaptive period and during study. During this adaptive period, volunteers will also be familiarized with exercises techniques and overload patterns that will be used throughout the study through 4 preparatory sessions.

4. At the end of the adaptation period, participants will participate in assessment procedures (baseline testing) at University facilities. Fasting blood samples will be collected by venipuncture using a disposable needle (20-gauge) in order to etsimate the lipid profile (TCHO, LDL, HDH, TG), fasting glucose and insulin, Hemoglobin (HbA1c) test in order to measure the amount of blood sugar (glucose) attached to hemoglobin and HOMA-IR, muscle damage markers (CK), inflammation markers (WBC and GRA), oxidative stress markers (PC, GSH, GSSG, ΤΑC and CAT), cortisol, blood test (HGB, HCT, LYM, MON, PLT and RBC) and cytokines indicators (IL-1b, IL-6 and CRP). The following will be included in the assessment procedures: Body weight (BW) and Height, body mass index (BMI), Body composition (DXA method), the circumferences of waist and hip in order to estimate the waist hip ratio (WHR). Also, the volunteers will participate in health-related measurements as: Bone mineral density, Bone mineral content, Hip structural analysis, Arterial blood pressure- Mean arterial blood pressure, resting heart rate, ultrasonography for heart and blood vessels and functional movement screen (FMS). The performance measurements in baseline testing are included the following procedures: maximal oxygen consumption and heart rate maximum (Vo2max-HRmax prediction using the Balke test), strength (1- repetition maximum in leg press and chest press), the knee-hip and ankle range of motion will be determined using a goniometer, the balance and gait speed indicator will be determine using Romberg's test and short physical performance battery tool respectively. Also, muscle endurance tests will be used and flexibility will be measured using sit and reach test. 10m and 30m sprint test will be measured in the same period using photocells device and the conter movement jump will be measured using a force platform. Measurements of well-being (vitality, quality of life, flow, depression, behavioral regulations in exercise and pgycological well being) will be performed using modified questionnaires.

5. After the adaptive period all participants will be randomly assigned to four groups (control, 1 session/week, 2 sessions/week, 3 sessions/week). The footbal training that will be used throughout the 6 months intervention will be consisted of wurm up (7-10 minutes), Tecnhical drills (5-8 minutes), fitness exercises (5-10 minutes), football games (30 minutes) and recovery period (5 minutes). During the sessions will be assessed the change in Heart rate using a heart rate monitor and the field activity will be recorded using a global positiong system (GPS). At the end of every session will be used Borg scale in monitoring progress and mode of exercise. Also, following a single training session both at pre and post training will be measured the delayed onset muscle soreness scale (DOMS), the Creatine Kinase activity, the isometric force of knee extensors and knee flexors, the eccentric and concentric peak torque using isokinetic dynamometer, the counter movement jumb ability and blood samples will be collected in order to estimate biochemical indicators (Oxidative stress and inflammatory response). Fianlly, the energy expenditure during training will be measured via blood lactate and oxygen consumption.

6. After 6 months of football intervention all participants will participate in assessment procedures (post-training testing) at University facilities within 5 days after the completion of the last training session.

All participants will be randomly assigned to the following four groups:

1. Control group (no training)

2. FIM-1 (1 session/week)

3. FIM-2 (2 sessions/week)

4. FIM-3 (3 sessions/week)

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
48
Inclusion Criteria
  1. The participants Vo2max <30ml/kg/min
  2. age of 40-60 years
  3. medical clearance for strenuous physical training
  4. Free of chronic diseases
  5. Free of musculoskeletal injury
  6. waist circumference 80≥ cm for female and 94≥ cm for Male participants
  7. no weight loss greater >10% of body mass before (≤6 months) the study
  8. no diet intervention or usage of nutritional supplements/medications before (≥6 months) and during the study

Additionaly the participants will have at least two of the following risk factors

  • overweight/obese (BMI 25.0-35.0)
  • Fasting Glucose≥100 mg/dl
  • Blood Pressure≥120/80 mmHg
  • Total Cholesterol≥190 mg/dl
  • High Desnity lipoprotein< 35 mg/dl for male and < 39 mg/dl for female participants
  • Low Density Lipoprotein≥100 mg/dl
  • Triglycerides≥150 mg/dl
Exclusion Criteria

Participants will be excluded from the study if they:

  1. will not participate in ≥80% of total exercise sessions
  2. will adhere to a nutritional intervention during the study
  3. will modify the habitual physical activity levels during the study
  4. Musculoskeletal injury
  5. Chronic disease

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
FIM-1FIM-1Participated in a supervised 6 months football training program once per week and in measurements at baseline and at 6 months.
FIM-2FIM-2Participated in a supervised 6 months football training program twice per week and in measurements at baseline and at 6 months.
ControlControlNo intervention. Participated only in measurements at baseline and at 6 months.
FIM-3FIM-3Participated in a supervised 6 months football training program thrice per week and in measurements at baseline and at 6 months.
Primary Outcome Measures
NameTimeMethod
Change in White blood cells (WBC)At baseline and at 6 months

White blood cells will be measured using an automatic blood analyzer

Change in Body mass indexAt baseline and at 6 months

Body mass index wil be calculated using the Quetelet's equation

Change in Body fatAt baseline and at 6 months

Body fat (%) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)

Change in resting metabolic rate (RMR)At baseline, at 6 months and 24 hours, 48 hours and 72 hours after

RMR (kcal) will be measured using a portable open-circuit indirect calorimeter with a ventilated hood system

Change in body mass content (BMC)At baseline and at 6 months

BMC (g) will be assessed by dual energy X-ray absorptiometry (DXA) of the total body, hip of non-dominant and dominant limb, spine and wrist

Change in Waist circumferenceAt baseline and at 6 months

Waist circumference (cm) will be measured using a Gullick II tape

Change in maximal strength (1RM)At baseline and at 6 months

1RM (kg) for the lower body will be measured bilaterally on a horizontal leg press, while 1RM (kg) for the upper body will be measured on a horizontal chest press

Change in dietary intakeAt baseline and at 6 months

Dietary intake (kcal) will be assessed using 7-day recalls

Change in maximal heart rate (MaxHR)At baseline and at 6 months

MaxHR will be estimated using a low-risk submaximal treadmill walking test

Change in Interleukin 6 (IL-6)At baseline and at 6 months

IL-6 (pg/ml) will be measured with commercially available kits

Change in oxidized glutathione (GSSG)At baseline and at 6 months

GSSG (nmol/L) will be measured in red blood cells with commercially available kits

Change in red blood cells (RBC)At baseline and at 6 months

RBC concentration will be measured using an automatic blood analyzer

Change in Lymphocytes (LYM)At baseline and at 6 months

LYM concentration will be measured using an automatic blood analyzer

Change in Hemoglobin A1c (HbA1c)At baseline and at 6 months

HbA1c concentration will be measured with commercially available kits

Change in Hip circumferenceAt baseline and at 6 months

Hip circumference (cm) will be measured using a Gullick II tape

Change in maximal oxygen consumption (VO2max)At baseline and at 6 months

VO2max (ml/kg/min) will be estimated using a low-risk submaximal treadmill walking test

Change in daily physical activityAt baseline and at 6 months

Daily physical activity will be assessed accelerometry device

Change in body mass density (BMD)At baseline and at 6 months

BMD (g) will be assessed by dual energy X-ray absorptiometry (DXA) of the total body, hip of non-dominant and dominant limb, spine and wrist

Change in knee, hip and ankle range of motionAt baseline and at 6 months

Knee, hip and ankle range of motion will be assessed using manual goniometer

Change in flexibilityAt baseline and at 6 months

Flexibility (cm) will be assessed using the modified sit-and-reach test

Change in Interleukin 1 beta (IL-1b)At baseline and at 6 months

IL-1b (pg/ml) will be measured with commercially available kits

Change in fasting blood glucose (FBG)At baseline and at 6 months

FBG (mg/dL) will be measured with commercially available kits

Change in Uric acid (UA)At baseline and at 6 months

UA concentration will be measured in plasma with commercially available kits

Change in isometric knee flexors peak torqueAt baseline, at 6 months and 24 hours, 48 hours and 72 hours after

isometric knee flexors peak torque will be assessed on an isokinetic dynamometer

Change in eccentric knee extensors peak torqueAt baseline and at 6 months

eccentric knee extensors peak torque will be assessed on an isokinetic dynamometer

Change in counter movement jump flight time (CMJf)At baseline, at 6 months and 24 hours, 48 hours and 72 hours after

CMJf (ms) will be assessed using a force platform with each foot parallel on platform

Change in fat massAt baseline and at 6 months

Body fat (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)

Change in fat-free massAt baseline and at 6 months

Fat-free mass (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)

Change in functional mobilityAt baseline and at 6 months

Functional mobility (sec) will be assessed using Gait speed test

Change in homeostatic model assessemnet for insulin resistance (HOMA-IR)At baseline and at 6 months

HOMA-IR will be measured with commercially available kits. ΗΟΜΑ score will be calculated using the equation HOMA-IR = fasting insulin (mIU/L) x fasting glucose (mg/dL) / 405. HOMA-IR score will be classified using the following range: normal insulin resistance \< 3, moderate insulin resistance 3-5, severe insulin resistance \> 5)

Change in Protein Carbonyls (PC) concentrationAt baseline and at 6 months

PC (mg) will be measured in red blood cells with commercially available kits

Change in C - reactive protein (CRP)At baseline and at 6 months

CRP (mg/L) will be measured with commercially available kits

Change in Granulocyte (GRA)At baseline and at 6 months

GRA concentration will be measured using an automatic blood analyzer

Change in Platelet (PLT)At baseline and at 6 months

PLT concentration will be measured using an automatic blood analyzer

Change in monocytes (MON)At baseline and at 6 months

PLT concentration will be measured using an automatic blood analyzer

Change in body massAt baseline and at 6 months

Body mass (kg) wil be measured using a beam balance

Change in Waist-to-hip ratioAt baseline and at 6 months

Waist-to-hip ratio will be calculated by dividing the waist by the hip measurement

Change in hip structural analysisAt baseline and at 6 months

hip structural analysis will be assessed by dual energy X-ray absorptiometry (DXA) of the total body, hip of non-dominant and dominant limb, spine and wrist

Change in blood lipidsAt baseline and at 6 months

Total serum cholesterol (mmol/L), triglycerides (mmol/L), low density lipoprotein (mmol/L) will be measured with commercially availlable kits.

Change in muscle enduranceAt baseline and at 6 months

Muscular endurance (repetitions until muscle failure) will be assessed using timed tests (60 sec) for the abdominal musculature, upper and lower body. The tests will include partial curl-up, push-up for males and modified push-up for females (kneeling position) respectively.

Change in glutathione (GSH)At baseline and at 6 months

GSH (nmol/L) will be measured in red blood cells with commercially available kits

Change in resting systolic (SBP) and diastolic (DBP) blood pressuresAt baseline and at 6 months

Resting SBP (mmHg) and DBP (mmHg) will be assessed by a manual sphygmomanometer

Change in mean arterial pressure (MAP)At baseline and at 6 months

MAP (mmHg) will be calculated using the following equation : MAP = SBP + (DBP × DBP) / 3

Change in resting heart rate (RHR)At baseline and at 6 months

RHR will be measured by pulse palpation for 60 seconds

Change in functional capacityAt baseline and at 6 months

Functional capacity will be assessed using a movement-based screening tool titled Functional Movement Screening (FMS). The FMS will be consisted of 7 movement tasks that will be scored from 0 to 3 points and the sum will create score ranging from 0 to 21 points (0 = pain with pattern regardless of quality, 1 = unable to perform pattern, 2 = able to perform pattern with compensation/imperfection, 3 = able to perform pattern as directed).

Change in static balanceAt baseline and at 6 months

Static balance will be assessed using the Sharened Romberg test

Change inTotal antioxidant capacity (TAC)At baseline and at 6 months

TAC will be will be measured with commercially available kits

Change in CortisolAt baseline and at 6 months

Cortisol (nmol/L) will be measured with commercially available kits

Change in insulinAt baseline and at 6 months

Insulin (mlU/L) will be measured with commercially available kits

Change in Catalase (CAT) activityAt baseline and at 6 months

CAT activity (units) will be measured in red blood cells with commercially available kits

Change in eccentric knee flexors peak torqueAt baseline and at 6 months

eccentric knee flexors peak torque will be assessed on an isokinetic dynamometer

Change in concentric knee flexors peak torqueAt baseline and at 6 months

concentric knee flexors peak torque will be assessed on an isokinetic dynamometer

Change in counter movement jump height (CMJh)At baseline, at 6 months and 24 hours, 48 hours and 72 hours after

CMJh (cm) will be assessed using a force platform with each foot parallel on platform

Change in sprint time of 10mAt baseline, at 6 months and 24 hours, 48 hours and 72 hours after

Sprint time (sec) will be assessed over a 10 m distance ising light cells

Change in Hematocrit (HCT)At baseline and at 6 months

HCT concentration will be measured using an automatic blood analyzer

Change in Hemoglobin (HGB)At baseline and at 6 months

HGB concentration will be measured using an automatic blood analyzer

Change in Cratine Kinase (CK) concentrationAt 6 months and 24 hours, 48 hours and 72 hours after

CK concentration will be measured with commercially available kits

Change in concentric knee extensors peak torqueAt baseline and at 6 months

concentric knee extensors peak torque will be assessed on an isokinetic dynamometer

Change in left ventricular end-diastolic volume (LVEDV)At baseline and at 6 months

LVEDV (ml) will be measured using echocardiography

Change in interventicular septum and diastole (IVSd)At baseline and at 6 months

IVSd will be measured using echocardiography

Change in isometric knee extensors peak torqueAt baseline, at 6 months and 24 hours, 48 hours and 72 hours after

isometric knee extensors peak torque will be assessed on an isokinetic dynamometer

Change in counter movement jump power (CMJp)At baseline, at 6 months and 24 hours, 48 hours and 72 hours after

CMJp (w/kg) will be assessed using a force platform with each foot parallel on platform

Change in left ventricular stroke volume (LVSV)At baseline and at 6 months

LVSV (ml) will be measured using echocardiography

Change in left ventricular internal diameter and diastole (LVIDd)At baseline and at 6 months

LVIDd (mm) will be measured using echocardiography

Change in pulmonary artery systolic pressure (PASP)At baseline and at 6 months

PASP (mmHg) will be measured using echocardiography

Change vitalityAt baseline and at 6 months

Vitality will be assessed using Subjective Vitality Scales (SVS) via 6 items. Each item is rated on a 6-point scale (1 = not at all true, 2 = not true, 3 = almost not true, 4 = almost true, 5 = true, 6 = very true). The total score ranges from 6 to 36 with a higher score indicating a better condition.

Change in sprint time of 30mAt baseline, at 6 months and 24 hours, 48 hours and 72 hours after

Sprint time (sec) will be assessed over a 30 m distance ising light cells

Change in interventicular septum and systole (IVSs)At baseline and at 6 months

IVSs will be measured using echocardiography

Change in left ventricular mass (LV mass)At baseline and at 6 months

LV mass (g) will be measured using echocardiography

Change in aortic valve velocity (AoV Vel)At baseline and at 6 months

AoV Vel (cm/s) will be measured using echocardiography

Change in left ventricular end-systolic volume (LVESV)At baseline and at 6 months

LVESV (ml) will be measured using echocardiography

Change in left ventricular ejection fraction (LVEF)At baseline and at 6 months

LVEF (%) will be measured using echocardiography

Change in left ventricular internal diameter and systole (LVIDs)At baseline and at 6 months

LVIDs (mm) will be measured using echocardiography

Change in aortic rootAt baseline and at 6 months

Aortic root (mm) will be measured using echocardiography

Change in right ventricular end diastole 1 (RVD1)At baseline and at 6 months

RVD1 (mm) will be measured using echocardiography

Change in tricuspid annular systolic velocity (TV S') of Right ventricular functionalityAt baseline and at 6 months

TV S' (mm) will be measured using echocardiography

Change in left ventricular fractional shortenning (FS)At baseline and at 6 months

Fractional shortenning (%) will be measured using echocardiography

Change in left ventricular Global Longitudinal strain (GLS)At baseline and at 6 months

GLS (%) will be measured using echocardiography

Change in pshycological well - beingAt baseline and at 6 months

Psychological well - being will be assessed using The General Health Questionnaire (GHQ-12) via 12 items

Change in motivationAt baseline and at 6 months

E xercise behavioral will be assessed using the behavioral regulation in (BREQ-2) via 19 items questionnaire. All the 19 items were positive scored, and it was rated on a five-point for each item from 0 (not true for me) to 4 (very true for me) to identify what the participants felt about exercise.

Change in flowAt baseline and at 6 months

Flow will be assessed using the Kurz Skala questionnaire. The Kurzskala consists 16 items which has to be answered on a seven-point rating scale from "does not apply" to "somewhat partially" to "applies".

Change in left atrial (LA) diameterAt baseline and at 6 months

LA diameter (mm) will be measured using echocardiography

Change in Tricuspid Annular Plane Systolic Excursion of right ventricular functionality (TAPSE)At baseline and at 6 months

TAPSE (mm) will be measured using echocardiography

Change in depressionAt baseline and at 6 months

Depression will be assessed using the Patient Health Questionnaire (PHQ-9), which is a self-administered instrument consisiting of 9 multiple choice questions scored from 0 to 3. Higher total scores indicate higher depression severity.

Change in left ventricular posterior wall end diastole (LVPWd)At baseline and at 6 months

LVPWd (mm) will be measured using echocardiography

Change in aortic valve pressure gradient (AoV PG)At baseline and at 6 months

AoV PG (mmHg) will be measured using echocardiography

Change in right ventricular end diastole (RVD)At baseline and at 6 months

RVD (mm) will be measured using echocardiography

Change in quality of lifeAt baseline and at 6 months

Quality of life will be assessed using the physical and mental component subscales of the Greek 36-item Short-Form Healthy Survey (SF-36). The scores on both component subscales of the SF-36 will range from 0 to 100, with higher scores indicating better health status while the minimal clinically important difference will be 2 points.

Secondary Outcome Measures
NameTimeMethod
Change in blood lactate concentration (BLa)At 6 months

BLa (mmol/L) concentraton will be measured in a microphotometer with commerdially available kits. Blood samples will be collected pre, mid and post execise session (single bout) at 3 minute post exercise

Change in Delay onset muscle soreness (DOMS)At baseline, at 6 months and 24 hours, 48 hours and 72 hours after

DOMS will be assessed using a scale consisiting of 9 multiple choices scored from 1 to 10

Body HeightAt baseline

Height (cm) will be measured using stadiometer

Electrocardiogram (ECG)At baseline

ECG will be recorded using the electrical heart signals by electrocardiogram device in order to examined the resting heart rate

Change in training internal load monitoringAt 6 months

Training internal load monitoring will be assessed using a pulse devises

Change in exercise - induced caloric expenditureAt 6 months

Measure using portable indirect calorimetry system. Οxygen consumption will be collected during exercise session

Change in training external load monitoringAt 6 months

Training external load monitoring will be assessed using GPS devise

Trial Locations

Locations (1)

University o Thessaly, School of Physical Education and Sports Science

🇬🇷

Tríkala, Greece

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