Enhancing Human Intestinal Absorption of Carotenoids and Bioconversion of Carotene to Vitamin A in the Presence of Hass Avocados
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Post-prandial levels of provitamin A and vitamin A
研究概览
简要总结
Vitamin A is necessary in the human diet. The form of vitamin A found in fruits and vegetables is not "active" and must be converted to the active form in the human body. However, information on the ability of humans to absorb and convert vitamin A to the active form is still lacking. In this study, the investigators will observe the absorption and conversion of vitamin A from orange tomato sauce and/or carrots after a meal with fat (from avocado fruit) and a meal without fat. The investigators will also test whether eating these foods might protect the blood against damage that could lead to heart disease.
详细描述
The primary objective of this study will be to demonstrate that adding avocados to a carotene rich meal will promote the absorption of provitamin A carotenoids and enhance the delivery of greater quantities of vitamin A. This objective will be accomplished by quantitation of the immediate post-prandial plasma concentrations of parent carotenoids and vitamin A metabolites after subjects consume a meal with or without avocado in combination with a serving of tomato sauce (containing nutritionally relevant amounts of beta-carotene) or carrots.
The secondary objective of this study will be to determine if higher levels of carotenoids and other antioxidants transported in the bloodstream will have a protective role in promoting cardiovascular health. This objective will be accomplished by testing the oxidation capacity of lipoprotein fractions before and after meal supplementation.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Total cholesterol (140 to 200 mg/dL)
- •BMI 17 to 30
- •Age 18-70 years
排除标准
- •Lactating, pregnant, or plan to be pregnant during study
- •Tobacco use (cigarettes or chewing tobacco)
- •Metabolic disease
- •Malabsorption disorders
- •History of cancer, esophageal, gastric, or intestinal ulcers
- •History of liver or kidney insufficiency or failure
- •Allergies to tomatoes or tomato products
- •Allergies to carrots
- •Allergies to avocados
- •Obesity (BMI>30)
- •Hypercholesterolemia (total cholesterol> 200mg/dL)
结局指标
主要结局
Post-prandial levels of provitamin A and vitamin A
时间窗: Nine post-prandial blood samples will be taken over twelve hours
The absorption of and conversion of provitamin A carotenoids into vitamin A will be measured after the consumption of a carotenoid-rich meal. The meal will be served both with and without avocado as a source of lipid.
次要结局
- post-prandial antioxidant status of blood(comparing baseline vs. 5 hour status)
研究者
Jessica Cooperstone
Research Scientist
Ohio State University