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Impact of Aerobic Exercise Training on Sympathetic Vasoconstriction and Vascular Function in Essential Hypertension

Not Applicable
Completed
Conditions
Hypertension,Essential
Interventions
Other: Training
Registration Number
NCT03778489
Lead Sponsor
University of Copenhagen
Brief Summary

Sympathetic nervous activity plays an important role in the development of hypertension and studies have shown that the pannexin-1 channel is involved in the signalling of the sympathetic activity to the vascular bed. The main project aim is to investigate the effects of 10 weeks of high intensity training on the effect of the sympathetic nervous activity on vascular function in individuals with essential hypertension. A secondary aim is to assess the role of essential hypertension and physical activity on vascular endothelial function,

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Sedentary men and women age 35 - 65 years
  • Resting blood pressure >140/90 mmHg or Resting blood pressure <140/90 mmHg
  • Essential hypertension
  • Non or exclusively antihypertensive medication
Exclusion Criteria
  • Hypertensive condition other than essential hypertension
  • Other chronical diseases
  • Smoking
  • Excess alcohol intake or drug abuse

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
HypertensiveTrainingMen and women in age-group 35-65 years Resting blood pressure of \>140/90 mmHg non or only anti-hypertensive medication
ControlTrainingMen and women in age-group 35-65 years Resting blood pressure of \<140/90 mmHg no medication
Primary Outcome Measures
NameTimeMethod
Blood pressureBefore and after 8 weeks of training

Changes in systolic and diastolic blood pressure

Secondary Outcome Measures
NameTimeMethod
Mitochondrial function in endothelial cellsBefore and after 8 weeks of training

Endothelial cell mitochondrial respiration is measured by High Resolution Respirometry

Platelet reactivityBefore and after 8 weeks of training

Platelet reactivity is assessed by multiplate aggregometry

Body CompositionBefore and after 8 weeks of training

Measured by Dual energy x-ray absorptiometry

Pannexin channel function assessed in vivoBefore and after 8 weeks of training

Pannexin channel function is assessed by measurement of vascular conductance with and without channel inhibition

Pannexin channel distribution in skeletal muscle tissueBefore and after 8 weeks of training

Pannexin channel distribution is assessed immunohistochemically in muscle samples

Sympathetic activityBefore and after 8 weeks of training

Direct measurement of Muscle Sympathetic Nervous Activity via microneurography

Reactive oxygen species formation in in endothelial cellsBefore and after 8 weeks of training

Endothelial cell production of reactive oxygen species is measured by High Resolution Respirometry

Maximal oxygen uptakeBefore and after 8 weeks of training

Direct Measurement of oxygen uptake during a graded test on cycle ergometer

Vascular functionBefore and after 8 weeks of training

Vascular conductance measured by ultrasound doppler during infusion of vasoactive substances

RheologyBefore and after 8 weeks of training

Platelet rheology. The viscoelastic measurements are based on attainment of the Gel Point (GP) from which the fractal dimension, df, is determined.

Endothelial cell characterizationBefore and after 8 weeks of training

Assessment of protein and mRNA-levels of proteins of relevance for endothelial function

Skeletal muscle characterizationBefore and after 8 weeks of training

Assessment of protein and mRNA-levels of proteins of relevance for skeletal muscle function

Trial Locations

Locations (1)

University of Copenhagen

🇩🇰

Copenhagen, Denmark

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