Can Including Genotype Information Increase the Effectiveness of Dietary Interventions? Vegetables Intake and Polymorphism TAS2R38 Gene by Healthy Adults
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Healthy Lifestyle
- Sponsor
- Poznan University of Life Sciences
- Enrollment
- 174
- Locations
- 1
- Primary Endpoint
- TAS2R38 polymorphism
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
Personalized nutrition is one of the most up to date trends in human nutrition and gains much interest of general public and scientists as well. Although we have gained some knowledge on gene-trait associations, the real effectiveness and usefulness of genotype-based nutritional recommendations is unknown. Many personalized nutrition companies are on the market today, some of them use personalized nutrition based on genotype analysis. For this reason, scientific basis of this approach should be clarified.
Our project can thus increase knowledge which can be applied in dietary counseling practice. Although we focus on increase vegetable and fruits intake, the study is designed as a proof of concept.
Detailed Description
In humans, the TAS2R38 receptor gene is responsible for differences in the perception of bitter taste. This gene codes for a G protein that is associated with a flavor receptor regulated by phenylthiocarbamide (PTC) and propylthiouracil (PROP) ligands, which by binding to the receptor determines the degree of bitter taste. Cruciferous vegetables contain glucosinolates and isothiocyanates, which resemble PTC and PROP and thereby affect their perception of bitter taste through the TAS2R38 regulated receptor. The polymorphism of this gene allows to distinguish three phenotypes: * insensitive to bitter taste \[bitter-non tasters\] * moderately sensitive to bitter taste \[intermediate-bitter tasters\] * sensitive to bitter taste \[bitter taster\] Previous studies have shown that people who are carriers of one PAV haplotype experience a bitter taste more than AVI / AVI homozygotes, which are less sensitive to bitter taste. Hence, the TAS2R38 gene polymorphism is associated with nutritional decisions, including choice of vegetables and coffee. Aim of the study is to verify effectiveness of the genotype based dietary intervention in people with or without polymorphism of TAS2R38 gene.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- •injuries, chronic diseases (e.g. diabetes, metabolic syndrome, cancer, hyperthyroidism), recent diet, pregnancy, breastfeeding, limited communication to the extent that no nutritional history can be carried out, eating disorders (according to nutritional history)
Outcomes
Primary Outcomes
TAS2R38 polymorphism
Time Frame: baseline
genotyping of polymorphism TAS2R38 gene (rsr713598, rs1726866, and rs10246939)
Vegetables intake
Time Frame: Baseline, 20 weeks
Change in frequency of vegatables intake within the group and between the groups; Block vegetables intake screener
Secondary Outcomes
- Fat Mass (FM) %(Baseline, 20 weeks)
- Fat free mass (FFM)(Baseline, 20 weeks)
- aspartate aminotransferase (ASPAT)(Baseline, 20 weeks)
- Waist circumference (WC)(Baseline, 20 weeks)
- Triglycerides (TG)(Baseline, 20 week)
- HDL Cholesterol (HDL-Chol)(Baseline, 20 weeks)
- Insulin (INS)(Baseline, 20 weeks)
- Body Mass (BM)(Baseline, 20 weeks)
- Total Cholesterol (TChol)(Baseline, 20 weeks)
- LDL Cholesterol(LDL-Chol)(Baseline, 20 weeks)
- Glucose (GLU)(Baseline, 20 weeks)
- Alanine transaminase (ALAT)(Baseline, 20 weeks)