Vegetables Intake and Polymorphism TAS2R38 Gene by Healthy Adults

Not Applicable

Completed

• Conditions: Healthy Lifestyle; Taste Disorders; Diet Habit
• Interventions: Behavioral: Control Group 2 general nutritional recommendations; Behavioral: Intervention genotype personalized nutritional recommendations; Behavioral: Control Group 1 personalized nutritional recommendations

• Registration Number: NCT04145453
• Lead Sponsor: Poznan University of Life Sciences

Brief Summary

Personalized nutrition is one of the most up to date trends in human nutrition and gains much interest of general public and scientists as well. Although we have gained some knowledge on gene-trait associations, the real effectiveness and usefulness of genotype-based nutritional recommendations is unknown. Many personalized nutrition companies are on the market today, some of them use personalized nutrition based on genotype analysis. For this reason, scientific basis of this approach should be clarified.

Our project can thus increase knowledge which can be applied in dietary counseling practice. Although we focus on increase vegetable and fruits intake, the study is designed as a proof of concept.

Detailed Description

In humans, the TAS2R38 receptor gene is responsible for differences in the perception of bitter taste. This gene codes for a G protein that is associated with a flavor receptor regulated by phenylthiocarbamide (PTC) and propylthiouracil (PROP) ligands, which by binding to the receptor determines the degree of bitter taste. Cruciferous vegetables contain glucosinolates and isothiocyanates, which resemble PTC and PROP and thereby affect their perception of bitter taste through the TAS2R38 regulated receptor.

The polymorphism of this gene allows to distinguish three phenotypes:

* insensitive to bitter taste \[bitter-non tasters\]

* moderately sensitive to bitter taste \[intermediate-bitter tasters\]

* sensitive to bitter taste \[bitter taster\] Previous studies have shown that people who are carriers of one PAV haplotype experience a bitter taste more than AVI / AVI homozygotes, which are less sensitive to bitter taste. Hence, the TAS2R38 gene polymorphism is associated with nutritional decisions, including choice of vegetables and coffee.

Aim of the study is to verify effectiveness of the genotype based dietary intervention in people with or without polymorphism of TAS2R38 gene.

Study Timeline

• Study Start: 2019-10-01
• Study First Submitted: 2019-10-03
• Study First Submitted QC: 2019-10-29
• Study First Posted: 2019-10-30
• Primary Completion: 2022-02-17
• Study Completion: 2022-02-17
• Status Verified: 2022-07
• Last Update Submitted: 2022-07-07
• Last Update Posted: 2022-07-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 174

Inclusion Criteria

• healthy

Exclusion Criteria

• injuries, chronic diseases (e.g. diabetes, metabolic syndrome, cancer, hyperthyroidism), recent diet, pregnancy, breastfeeding, limited communication to the extent that no nutritional history can be carried out, eating disorders (according to nutritional history)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Control group 2	Control Group 2 general nutritional recommendations	Participants with AVI haplotype will receive general recommendations regarding consumption of fruit and vegetables.
intervention group	Intervention genotype personalized nutritional recommendations	Participants with PAV haplotype will receive personalised dietary recommendations regarding consumption of fruit and vegetables. The information about genotype will be given at the beginning of study.
control group 1	Control Group 1 personalized nutritional recommendations	Participants with PAV haplotype will receive personalised dietary recommendations regarding consumption of fruit and vegetables, but the information about genotype will be given at the end of study.

Primary Outcome Measures

Name	Time	Method
TAS2R38 polymorphism	baseline	genotyping of polymorphism TAS2R38 gene (rsr713598, rs1726866, and rs10246939)
Vegetables intake	Baseline, 20 weeks	Change in frequency of vegatables intake within the group and between the groups; Block vegetables intake screener

Secondary Outcome Measures

Name	Time	Method
Fat Mass (FM) %	Baseline, 20 weeks	change in Fat Mass % within the group and between the groups
Fat free mass (FFM)	Baseline, 20 weeks	change in FFM (kg) within the group and between the groups
aspartate aminotransferase (ASPAT)	Baseline, 20 weeks	Changes in ASPAT (U/l) within the group and between the groups
Waist circumference (WC)	Baseline, 20 weeks	Change in WC (cm) within the group and between the groups
Triglycerides (TG)	Baseline, 20 week	Changes in TG (mg/dl) within groups and between groups
HDL Cholesterol (HDL-Chol)	Baseline, 20 weeks	Changes in HDL-Chol (mg/dl) within the group and between the groups
Insulin (INS)	Baseline, 20 weeks	Changes in INS (ulU/ml) within the group and between the groups
Body Mass (BM)	Baseline, 20 weeks	change in body mass (kg) within the group and between the groups
Total Cholesterol (TChol)	Baseline, 20 weeks	Changes in TChol (mg/dl) within the group and between the groups
LDL Cholesterol(LDL-Chol)	Baseline, 20 weeks	Changes in LDL-Chol (mg/dl) within the group and between the groups
Glucose (GLU)	Baseline, 20 weeks	Changes in GLU (mg/dl) within the group and between the groups
Alanine transaminase (ALAT)	Baseline, 20 weeks	Changes in ALAT (U/l) within the group and between the groups

Trial Locations

Locations (1)

Poznan University of Life Sciences; 🇵🇱 Poznań, Poland