Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men

Not Applicable

Completed

• Conditions: Skeletal Muscle; Amino Acids Metabolism; Exercise; Extracellular Matrix; Dietary Supplements
• Interventions: Dietary Supplement: Collagen Peptide; Dietary Supplement: Maltodextrin Placebo; Dietary Supplement: Essential Amino Acid

• Registration Number: NCT04596553
• Lead Sponsor: University of British Columbia

Brief Summary

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Detailed Description

This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study First Submitted: 2020-09-28
• Study First Submitted QC: 2020-10-15
• Study First Posted: 2020-10-22
• Study Start: 2021-01-25
• Primary Completion: 2022-02-13
• Study Completion: 2022-02-13
• Status Verified: 2023-06
• Last Update Submitted: 2023-06-09
• Last Update Posted: 2023-06-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 17

Inclusion Criteria

• Can fluently read and write in English
• Male
• 18 - 35 years

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Exclusion Criteria

• Diabetes, cardiovascular disease, or current pharmaceutical therapy
• Medical conditions impacting ability to undertake strenuous physical activity
• Lower body resistance training in the previous 6 months
• BMI > 30
• Currently smoking with cigarettes
• Allergy to any study supplements
• Currently taking any protein, amino acid, or collagen supplement
• Participate in greater than 2 hours of weekly vigorous exercise

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Collagen Peptide	Collagen Peptide	Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
Maltodextrin Placebo	Maltodextrin Placebo	Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
Essential Amino Acid	Essential Amino Acid	Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water

Primary Outcome Measures

Name	Time	Method
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis	2 - 4 hours post-exercise	Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
Fractional synthetic rate (change over time) Muscle Collagen Synthesis	2 - 4 hours post-exercise	Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
Pro-Collagen Type I N-Propeptide (PINP)	4 hours post exercise	Osteoblastic marker of bone collagen synthesis

Secondary Outcome Measures

Name	Time	Method
PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)	0 - 4 hours post-exercise	Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis
Muscle mechanistic target of rapamycin Pathway Activation	0 - 4 hours post-exercise	Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt))
Pro-Collagen Type III N-Propeptide (PIIINP)	4 hours post exercise	Marker of collagen synthesis
Plasma Amino Acids Response to Supplementation	0 - 4 hours post-exercise	Amino acid plasma concentrations

Trial Locations

Locations (1)

Chan Gunn Pavilion; 🇨🇦 Vancouver, British Columbia, Canada