Effects of Probiotic Ayran on Gingivitis
- Conditions
- GingivitisProbioticsInflamed Gums
- Interventions
- Dietary Supplement: probiotic ayran drinkDietary Supplement: Placebo ayran drink
- Registration Number
- NCT06437925
- Lead Sponsor
- Cukurova University
- Brief Summary
Objectives: This study investigates the effects of daily consumption of probiotic ayran drink on gingival inflammation and the development of experimental gingivitis.
Methods: A total of 54volunteer students were included in the present randomized, double-blind, placebo-controlled trial.The participants were divided randomly into two groups; The Control group consisted of 27 participants who consumed placebo ayran, while the 27 participants of the Test group consumed probiotic ayran (containing Lactobacillus acidophilus and Bifidobacterium bifidum) for 42 days twice a day.After 42 days, mechanical plaque control was interrupted for 5 days. The clinical parameters of gingivitis; Plaque index (PI), gingival index (GI), probing bleeding (BOP), probing depth (PPD) were recorded at baseline, day 42 (beginning of experimental gingivitis) and day 47 (the end of experimental gingivitis). At the same time points, gingival crevicular fluid had been collected for analysis of matrix metalloproteinase - 8 (MMP-8).
- Detailed Description
The most used and studied probiotics in the periodontal literature are lactobacillus and bifidobacterium. The current study aims to observe the effects of daily ayran consumption containing a probiotic combination of Lactobacillus acidophilus and Bifidobacterium bifidum on plaque development and gingival inflammation in an experimental gingivitis model of healthy individuals. The null hypothesis of this study using of probiotics has no additional benefit for periodontal health.
This study evaluates the effects of daily consumption of ayran containing a probiotic combination of Lactobacillus Acidophilus and Bifidobacterium Bifidum on plaque development and gingival status in healthy individuals with experimental gingivitis.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 54
- Systemic healthy
- Subjects with gingivitis defined as a BOP sites ≥ 10% and PD ≤ 3 mm
- No radiographic bone loss
- Non-smoking participants
- History of using antibiotics or anti-inflammatory drugs or probiotic preparations or food supplements in the last 6 months,
- Undergoing orthodontic treatment,
- Active carious lesions
- Mouth breathing
- History of allergy for milk or fermented milk products.
- Taking medications affecting the gingiva and/or oral mucosa
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Probiotic ayran drink probiotic ayran drink The participants in this experimental group will use probiotic containing ayran for 42 days and then will be subjected to 5 days of experimental gingivitis. Placebo ayran drink Placebo ayran drink The participants in this control group will use control ayran without probiotics for 42 days and then will be subjected to 5 days of experimental gingivitis.
- Primary Outcome Measures
Name Time Method Plaque index Plaque index will be recorded at baseline, at day 42 and 47 PI was scored from 0 to 5 according to the color change obtained with the Mira-2 solution
Gingival index Gingival index will be recorded at baseline, at day 42 and 47 GI is graded by visual assessment and mechanical stimulation of the gingival tissues, scoring the gingival condition according to the criteria
Bleeding on probing BOP will be recorded at baseline, at day 42 and 47 The probing bleeding (BOP) index was determined by the presence/absence of bleeding ≈30 seconds after probing
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Cukurova University Faculty of Dentistry
🇹🇷Adana, Turkey