Occupational exposure to waste anesthetic gases: evaluation of toxic effects in genetic material and antioxidant system in recently exposed professionals

Not Applicable

• Conditions: Occupational exposure, waste anesthetic gases, genotoxicity, oxidative stress; N06.850.460.350.600; D27.505.696.277.100.035.060; SP4.011.087.693.339; G03.495.710

• Registration Number: RBR-78m24n
• Lead Sponsor: Faculdade de Medicina de Botucatu - UNESP

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2014-11-07
• Last Update Posted: 29 May 2023

Recruitment & Eligibility

• Status: Recruitment completed
• Sex: Not specified
• Target Recruitment: Not specified

Inclusion Criteria

Medical residents; young; 25 to 35 years of age; healthy; of both genders; with or without exposure to waste anesthetic gases.

Exclusion Criteria

Medical residents with neoclassic disease; inflammatory or infectious conditions; smokers; alcoholics; history of drug abuse; use of medication or antioxidant supplementation; recent exposure to radiation.

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The experimental group exposed to waste anesthetic gases will have a significant increase of DNA damage, measured by the Comet Assay, in which is expected to find a minimal increase of 10% in damaged lymphocytes per 100 cells in the exposed group opposed to the control group.

Secondary Outcome Measures

Name	Time	Method
The experimental group is expected to show an increase of inflammatory status compared to the control group (increase in 5 to 10% in pro-inflammatory cytokines), measured by the concentration of cytokines in plasma using flow cytometry.;The surgical rooms with air scavenging system are expected to have reduced concentration of waste anesthetic gases then the rooms without a scavenging system, measured with a infrared portable spectophotometer. These values are expected to be below the limits proposed by the National Institute of Occupational Safety and Health (NIOSH, 1994), which are 25ppm for N2O, 2ppm for halogenated agents and 0,5ppm for halogenated agents used with N2O.;The experimental group exposed to waste anesthetic gases will have a significant increase of 7-10% in the concentration of Malondialdehyde (MDA) measured through High-performance liquid chromatography (HPLC) in contrast with the control group, which indicates a higher oxidative status in the exposed group.