The Molecular Pathogenesis Study of Cataract
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Cataract
- Sponsor
- Eye & ENT Hospital of Fudan University
- Enrollment
- 150
- Locations
- 1
- Primary Endpoint
- m6A modification of mRNAs
- Status
- Recruiting
- Last Updated
- 3 years ago
Overview
Brief Summary
To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients
Detailed Description
Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.
Investigators
Jin Yang
Professor
Eye & ENT Hospital of Fudan University
Eligibility Criteria
Inclusion Criteria
- •diabetic cataract
- •underwent cataract surgery
Exclusion Criteria
- •Combined with other eye diseases
- •underwent other eye surgery
Outcomes
Primary Outcomes
m6A modification of mRNAs
Time Frame: 1 month
Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples
levels of RNA methyltransferase
Time Frame: 1 month
in vitro validation of the diverse expression levels of RNA methyltransferase
expression level of mRNAs
Time Frame: 1 month
the m6A microarray analyses provide data for mRNA expression levels
levels of methylated mRNA
Time Frame: 1 month
in vitro validation of the diverse expression levels of methylated mRNA