The Molecular Pathogenesis Study of Cataract

Recruiting

• Conditions: Cataract; Pathogenesis

• Registration Number: NCT05682001
• Lead Sponsor: Eye & ENT Hospital of Fudan University

Brief Summary

To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients

Detailed Description

Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.

Study Timeline

• Study Start: 2022-01-01
• Study First Submitted: 2022-12-06
• Status Verified: 2023-01
• Study First Submitted QC: 2023-01-03
• Last Update Submitted: 2023-01-03
• Study First Posted: 2023-01-12
• Last Update Posted: 2023-01-12
• Primary Completion: 2023-12-01
• Study Completion: 2025-12-30

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 150

Inclusion Criteria

• diabetic cataract
• underwent cataract surgery

Exclusion Criteria

• Combined with other eye diseases
• underwent other eye surgery

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
m6A modification of mRNAs	1 month	Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples
levels of RNA methyltransferase	1 month	in vitro validation of the diverse expression levels of RNA methyltransferase
expression level of mRNAs	1 month	the m6A microarray analyses provide data for mRNA expression levels
levels of methylated mRNA	1 month	in vitro validation of the diverse expression levels of methylated mRNA

Trial Locations

Locations (1)

Eye & ENT Hospital of Fudan University; 🇨🇳 Shanghai, Shanghai, China