The Pathogenesis of Age-related Cataract
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Cataract
- Sponsor
- Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- Characterization of miRNA in micro-scale human aqueous humor
- Last Updated
- 7 years ago
Overview
Brief Summary
To study the pathological mechanism of cataract by genomics, transcriptomics, and proteomics.
Detailed Description
Tissue samples and aqueous humor samples will be collected from each individual patients by cataract surgery. After sample treatment, different techniques will be applied for analysis. And bioinformatics approach is applied for data treatment and analysis.The data of genomics, transcriptomics, and proteomics will be analyzed for study of pathological mechanism of cataract.
Investigators
Haidong Zou
Professor
Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
Eligibility Criteria
Inclusion Criteria
- •Patients with the explicit diagnosis of cataract (including senile cataract, congenital cataract, complicated cataract, metabolic cataract, traumatic cataract, etc.) in the operated eye are willing to undergo cataract surgery.
Exclusion Criteria
- •Patients whose cataracts need not be operated
- •Patients with any contraindications to cataract surgery
- •Patients are unwilling to undergo cataract surgery
Outcomes
Primary Outcomes
Characterization of miRNA in micro-scale human aqueous humor
Time Frame: 12 month
method development for miRNA detection in aqueous humor
Study of cataract by genomics, transcriptomics, and proteomics approaches
Time Frame: 36 month
detect histone H3 lysine 4 trimethylation (H3K4me3) of aqueous humor of cataract patients by genomics, transcriptomics, and proteomics methods
Favored amplification recovery via protection-chromatin immunoprecipitation-Seq of human eye tissue samples
Time Frame: 24 month
low-cell-number epigenome profiling for study of cataract