Effects of Aerobic and Resistance Training on Accumulation of Old, Modified Proteins in Young and Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- Mayo Clinic
- 入组人数
- 72
- 试验地点
- 2
- 主要终点
- Skeletal muscle protein synthesis rate
研究概览
简要总结
Muscle proteins accumulate damage during aging and leads to the loss of muscle mass and function in older people. Exercise can increase the making of new proteins and removal of older proteins, but it is not known if the effect changes with aging or type of exercise. The investigators will determine the ability for endurance, resistance, or a combination of exercise training to remove older-damaged proteins and make newer-functional muscle proteins in groups of younger and older people. The investigators will particularly study protein that are involved with energy production (mitochondrial proteins) and force production (contractile proteins).
Hypothesis 1: Older people will have greater accumulation of damaged proteins than younger people.
Hypothesis 2: Aerobic exercise will decrease the accumulation of damaged forms of contractile and mitochondrial proteins in younger and older people.
Hypothesis 3: Resistance exercise will decrease the accumulation of damaged forms of contractile proteins in younger and older people.
详细描述
The loss of muscle mass and function with age leads to high social and economic costs. Lifestyle interventions that can help maintain muscle mass and function can be beneficial to improve health and decrease the costs associated with loss of independence in the elderly. Muscle proteins accumulate damage during aging, which is suggested to lead to loss of function. The biological processes that remove damaged proteins and synthesis new proteins appear to be decreased with aging. Exercise is known to increase the processes that remove older and synthesis newer muscle proteins and may be an effect lifestyle intervention to improve muscle quality and function. Additionally, specific types of proteins appear to decay with age including contractile and mitochondrial proteins. Different types of exercise training can increase the making of specific proteins. The investigators will examine the ability for aerobic and resistance training to increase the quality of mitochondrial and contractile proteins between younger and older people.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •18 to 30 years or 65 to 80 years old
- •Male and female
排除标准
- •Regular exercise program
- •Metabolic disease (diabetes, cardiovascular disease, cancer, thyroid disorders)
- •Pregnancy
- •Inability to exercise
- •Overweight or obesity
- •Drugs known to impair metabolic function (statin, beta-blocker, anti-inflammatory)
- •Allergies to lidocaine
研究组 & 干预措施
Aerobic Exercise Training
Participants will perform 12-weeks of high intensity aerobic training.
干预措施: High intensity aerobic exercise (Behavioral)
Combined
The combined group will have 12-weeks of no exercise followed by 12-weeks of combined aerobic and resistance exercise training. Assessments will be made at three time points: baseline, after 12-weeks of no training, and after 12-weeks of combined training.
干预措施: Combined (Behavioral)
Resistance Exercise Training
Participants will perform 12-weeks of resistance exercise training.
干预措施: Resistance exercise training (Behavioral)
结局指标
主要结局
Skeletal muscle protein synthesis rate
时间窗: Approximately 14 weeks for the endurance or resistance training groups and approximately 28 weeks for the combined group
The investigators will determine the rate of incorporation of stable isotope amino acid tracers in skeletal muscle proteins during several hours of rest. The measurement will be an average resting muscle protein synthesis rate (% new muscle protein per hour) and will be performed at baseline and following 12 weeks of exercise training.
次要结局
未报告次要终点
研究者
K. Sreekumaran Nair
Principal Investigator
Mayo Clinic