The Effect of Remote Ischemic Conditioning on 2,3-bisphosphoglycerate in Erythrocyte
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 40
- 主要终点
- Changes of 2,3-BPG levels in erythrocyte
研究概览
简要总结
Under hypoxia or high-altitude hypoxia ,it has been found that 2,3-bisphosphoglycerate(2,3-BPG) in erythrocyte increased and facilitated oxygen release to tissues.Remote Ischemic Conditioning(RIC) can also cause hypoxic conditions in local limbs.Thus,we hypothesize that whether RIC could also increase 2,3-BPG in erythrocyte.
详细描述
RIC is induced by short-term insulting blood-flow of nonvital distant organs intermittently and has been proved to be effective in ischemic stroke and myocardial infarction(MI).The possible mechanisms of RIC protection is complicated.RIC leads to local ischemia and hypoxia and has been demonstrated to play a role in vital organs through hypoxia inducible factors(HIF) against stroke and MI.Besides HIF, hypoxia or high-altitude hypoxia could also change glycolytic process in erythrocyte through adenosine or sphingosine-1-phosphate(S1P).Accompanying with alternating glucolysis, 2,3-diphosphoglycerate(2,3-BPG) in erythrocyte increases and it also facilitating oxygen release to tissues.Thus,we hypothesize that whether RIC could also increase 2,3-BPG in erythrocyte.There are 8 arms in this trial: All arms are RIC treatment. The level of 2,3-BPG in erythrocyte will be assessed by ELISA before and after the treatment.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Investigator)
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age:18-65 years old
- •Healthy adults
排除标准
- •Individuals with various chronic diseases.
- •Individuals with various acute diseases.
- •Individuals who take any medicines
- •Individuals who have taken health supplements for more than 2 weeks.
- •individuals with BMI<18.5 or BMI≥24
结局指标
主要结局
Changes of 2,3-BPG levels in erythrocyte
时间窗: through study completion, an average of 24 hours
Whole blood for testing effect of RIC on 2,3-BPG in Erythrocyte
次要结局
- Changes of hypoxia inducible factor-1(HIF-1)(through study completion, an average of 24 hours)
- Changes of activated partial thrombin time(TT)(through study completion, an average of 24 hours)
- Changes of lower limbs venous blood flow(through study completion, an average of 24 hours)
- Changes of D-dimer(through study completion, an average of 24 hours)
- Changes of activated partial thromboplastin time(APPT)(through study completion, an average of 24 hours)
- Changes of prothrombin time(PT)(through study completion, an average of 24 hours)
- Changes of Fibrinogen(FIB)(through study completion, an average of 24 hours)
研究者
Ji Xunming,MD,PhD
Professor
Capital Medical University