Phenotypic and Genotypic Characteristics of Pseudomonas Aeruginosa Isolates in Sohag University Hospitals

Recruiting

• Conditions: Pseudomonas Aeruginosa Infection

• Registration Number: NCT06417593
• Lead Sponsor: Sohag University

Brief Summary

Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions from Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections that will be obtained under complete aseptic precautions.

Detailed Description

Not available

Study Timeline

• Study Start: 2024-04-01
• Status Verified: 2024-05
• Study First Submitted: 2024-05-11
• Study First Submitted QC: 2024-05-11
• Study First Posted: 2024-05-16
• Last Update Submitted: 2024-05-16
• Last Update Posted: 2024-05-20
• Primary Completion: 2024-11-01
• Study Completion: 2025-03-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Samples from patients with different types of health care associated infections as urinary tract infections, infected burn, ventilator associated pneumonia, blood stream infections and surgical site infections will be obtained under complete aseptic precautions.

Exclusion Criteria

• Any infection before 48 hours of patient admission at Hospital

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Detection of biofilm forming isolates	6 months	Detection of biofilm forming isolates by tissue culture plate method.
Identification of antibiotic susceptibility pattern of isolated strains of pseudomonas	6 months	Antibiotic susceptibility testing of the isolates will be performed using disc diffusion method according to clinical laboratory standards institute (CLSI) guidelines
Isolation and identification of pseudomonas aeruginosa causing different nosocomial infections.	6 months	Isolation and identification of Pseudomonas aeruginosa using basic microbiological methods, such as Gram staining, cultivation on cetrimide agar and biochemical reactions
Detection of virulence and antibiotic resistance genes of pseudomonas aeruginosa.	6 months	Detection of virulence and antibiotic resistance genes by conventional Polymerase Chain Reaction

Trial Locations

Locations (1)

Faculty of medicine Sohag university hospital; 🇪🇬 Sohag, Egypt