Research for markers of bone quality
Completed
- Conditions
- Osteoporosis10017322
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 100
Inclusion Criteria
Postmenopausal women with a vertebral fracture and postmenopausal women without a vertebral fracture with high bone mineral density (T-score >-1 S.D.)
Exclusion Criteria
Medication influencing osteoclast function and formation (Bisphosphonates, Hormone Replacement Therapy, Strontium Ranelate, Teriparatide, Raloxifen)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Osteoclast formation will be studied by staining them with a Tartrate Resistant<br /><br>Acid Phosphatase staining.<br /><br>Bone resorption will be studied using a pit-assay to determine the surface and<br /><br>the number of resorption pits.</p><br>
- Secondary Outcome Measures
Name Time Method <p>To study the expression of genetic markers of bone resorption and fractures,<br /><br>osteoclast will be seeded for RNA isolation. Furthermore, since osteoclast are<br /><br>derived from the hematopoietic line like lymfocytes, the PBMCs will be used to<br /><br>study the influence of lymfocytes and factors derived from lymfocytes on<br /><br>osteoclasts.<br /><br>Furhtermore blood will be collected as well to study biochemic and serum<br /><br>markers to be able to relate them to osteoporosis or other pathology.</p><br>