Fructose Rich Diet and Endothelial Function
- Conditions
- Metabolic DiseaseAerobic ExerciseFructose Rich DietFructose Metabolism Disorder
- Interventions
- Dietary Supplement: GlucoseDietary Supplement: FructoseOther: Fructose and exercise
- Registration Number
- NCT03939130
- Lead Sponsor
- Federal University of Rio Grande do Sul
- Brief Summary
Cardiometabolic diseases have been associated with high consumption of sweetened beverages. These products are responsible for the largest portion of the total consumption of fructose in the diet and it is suggested that excessive intake of this monosaccharide may contribute to the development of risk factors for these diseases due to differences in metabolism relative to glucose. However, there is a lack of data in the literature demonstrating the deleterious effects of excessive fructose consumption on vasodilation and whether aerobic training may be able to prevent or mitigate these damages in humans. Therefore, the aim of the study will be to verify the effect of 4 weeks of high fructose diet associated with aerobic training on uric acid levels and its influence on markers related to oxidative stress and vasodilatation. Twenty-one sedentary men and women, aged between 19 and 35 years, will be submitted to 4 weeks of intervention. In a randomized way, subjects will be divided into 3 groups: high fructose diet, high glucose diet and high fructose diet and exercise. Blood samples will be taken before, in the middle and after the intervention to verify the concentrations of uric acid, superoxide dismutase enzyme, thiobarbituric acid, nitrite / nitrate, lipid profile, glucose, insulin, C-reactive protein and endothelin-1. In addition, flow-mediated dilatation, insulin resistance index, pancreatic beta cell functional capacity index, oral glucose tolerance test, 24-hour blood pressure, heart rate variability and body composition will be analyzed. The comparisons will be performed through the Generalized Estimates of Equations, adopting the factors group and time. The Bonferroni post-hoc will be used to identify differences. The accepted level of significance will be 5%.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 21
- Men and women;
- Not engaged in regular exercises;
- Aged between 19 and 35 years;
- Body mass index (BMI) of less than 30kg / m².
- Individuals with BMI below 18kg / m² and greater than 30kg / m²;
- Smokers;
- Dyslipidemic;
- Diabetic;
- Hypertensive;
- Alcohol use;
- Appetite suppressants use;
- Medications use;
- Supplements use;
- Chronic disease that impedes the performance of physical exercise.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Glucose-rich diet Glucose Subjects will be submitted to a standard diet associated with a sweetened beverage (provided by the researchers) during 4 weeks. Without knowing the content (blind), they will receive glucose based beverage, prepared as a solution with 10% glucose, water and flavoring powder, totaling 1.0g / kg of body mass / day of glucose. Fructose-rich diet Fructose Subjects will be submitted to a standard diet associated with a sweetened beverage (provided by the researchers) during 4 weeks. Without knowing the content (blind), they will receive fructose based beverage, prepared as a solution with 10% fructose, water and flavoring powder, totaling 1.0g / kg of body mass / day of fructose. Fructose-rich diet and exercise Fructose and exercise The subjects will perform the same protocol described in the intervention Fructose-rich diet, except for the inclusion of the physical exercise. During the 4-week intervention, participants will perform three sessions a week of 60 minutes of aerobic exercise at 60% of VO2 peak on cycle ergometer.
- Primary Outcome Measures
Name Time Method Flow Mediated Dilatation 4 weeks Concentration of Nitrite / Nitrate 4 weeks Concentration of Uric Acid 4 weeks
- Secondary Outcome Measures
Name Time Method Concentration of Total Cholesterol 4 weeks Concentration of High-Density Lipoprotein 4 weeks Concentration of Substances Reactive to Thiobarbituric Acid 4 weeks Oxidative stress outcome
Concentration of Endothelin-1 4 weeks Concentration of Insulin 4 weeks Concentration of Superoxide Dismutase Enzyme 4 weeks Insulin Sensitivity 4 weeks Oral glucose tolerance test
Concentration of Triglycerides 4 weeks Concentration of Low-Density Lipoprotein 4 weeks HOMA-B 4 weeks 24-hour Blood Pressure 4 weeks Concentration of Glucose 4 weeks HOMA-IR 4 weeks Concentration of C-Reactive Protein 4 weeks Heart Rate Variability 4 weeks Body Fat 4 weeks
Trial Locations
- Locations (1)
Universidade Federal do Rio Grande do Sul
🇧🇷Porto Alegre, Rio Grande Do Sul, Brazil