T cell homeostasis after ATG induced lymphocytopenia in renal transplant patients; studied through in vivo 2H2O labeling
Completed
- Conditions
- low white blood cells1004795410018188lymphocytopenia10027665
- Registration Number
- NL-OMON35541
- Lead Sponsor
- Academisch Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 9
Inclusion Criteria
Renal transplantation patients treated with standard maintenance immunosuppressive therapy who were treated with ATG because of acute humoral graft rejection and renal transplantation patients treated with standard maintenance immunosuppressive therapy alone. Healthy volunteers, without relevant medical history.
Exclusion Criteria
Patients with intercurrent medical problems
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The amount of 2H2O incorporated into the DNA within the naïve CD4+ and CD8+ T<br /><br>cells will be measured. With special mathematical formulas the production rate<br /><br>and half-life of cells can be calculated. T cell homeostasis in the ATG treated<br /><br>patients, versus the standard immunosuppressive treated patients and versus the<br /><br>healthy volunteers will be analysed through comparing production rates and<br /><br>half-life of the naïve T cells. For that purpose we will use methods that have<br /><br>been described in the literature, and we will collaborate with these same<br /><br>investigators.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Production rates and half-life of the CD31+ CD4 naive T cells.</p><br>