MedPath

Growth Hormone Therapy for Muscle Regeneration in Severely Burned Patients

Phase 2
Completed
Conditions
Burns
Growth Hormone Treatment
Interventions
Drug: 0.09% Saline Solution
Registration Number
NCT03038594
Lead Sponsor
The University of Texas Medical Branch, Galveston
Brief Summary

The investigators have previously demonstrated that burn injury causes severe muscle wasting, weight and height retardation, and systemic protein catabolism in pediatric and adult burned patients. The persistent loss of muscle impairs the quality of life of the burned patients, and it also delays autonomy and reintegration into the community. In 2009, the investigators showed that the daily injection of recombinant human growth hormone (GH) for nine months post discharge significantly increased height and weight, as well as lean body mass, in pediatric burned subjects. Our long-term goal is to improve the quality of life of burn patients by preventing height, weight, and muscle loss that may occur from severe protein catabolism. The objectives of this application are to a) attenuate height and weight in burned patients with the administration of GH, b) prevent or reverse loss of muscle and strength in these patients, and c) collect pilot data about cardiopulmonary parameters, scar assessments, and muscle metabolism. Our central hypothesis is that the administration of GH will restore depleted levels of growth hormone and will lead to prevention of lean body mass loss and bone mineral content, improve rehabilitation, and accelerate reintegration of severely burned patients. The investigators will administer either placebo or GH (daily subcutaneous injections of 0.05 mg/kg/day of GH \[somatropin, Genotropin, Pfizer, New York, NY\] to adult burn subjects (n=31 per group, 18-85 years, \>30% total body surface burns) for nine months beginning one week prior to discharge. Both groups will be studied for a total of two years. The following aims will be tested: 1) determine the effects of GH supplementation on body composition, such as lean body mass loss, muscle strength, and exercise endurance; and 2) assess whether rehabilitation and subsequent reintegration of severely burned patients into society can be accelerated. Investigators will measure changes in lean body mass, muscle strength and exercise endurance during the acute hospital stay, discharge, and long-term follow-up visits (6, 12, 18, and 24 months after burn), as well as secondary endpoints such as cardiopulmonary variables, hypertrophic scar development, quality of life questionnaires, and concentrations of relevant hormones, cytokines, and oxidative stress markers.

Detailed Description

Either recombinant human growth hormone (daily subcutaneous injections of 0.05 mg/kg/day of GH at discharge \[somatropin, Genotropin, Pfizer, New York, NY\]; 0.025 mg/kg/day of GH titrated the week before discharge) or placebo (n=31) will be administered to adult burned subjects (n= 31, 18-85 years) after screening and voluntary consent who have ≥30% TBSA assessed by either the Lund and Browder chart or the 'rule of nines' method during excisional surgery. It will be administered daily for 9 months beginning the week before discharge, and the primary and secondary endpoints will be collected during the acute hospital stay, discharge, and long-term follow-up visits (6, 12, 18, and 24 months after burn injury). Additionally, subjects will be contacted frequently \[most likely 1 week, 1 month, and 2 months post discharge by telephone\] to ensure that there are no adverse events or concerns with their study drug, as well as visit with them during their clinical visits that address their post-burn needs. All subjects will receive similar standard medical care and treatment from the time of emergency admission until their discharge.

Growth hormone will be used to potentially attenuate losses in height, weight, muscle and bone, reverse the oxidative stress of burn injury and, in the process, decrease the secondary consequences of burn injury, including organ dysfunction. This may improve the quality of life of the burn patient by preventing pathophysiology that may result from muscle and bone loss and may reduce hospital stay. Our research will lay the foundation for the future development of effective, safe, and economic therapeutic interventions to treat burn injury-associated metabolic abnormalities. Also, it will provide the basis for the development of supplemental regulations and pharmacotherapy to treat burn patients with GH. The risks are very reasonable in relation to the anticipated benefits to our subjects because a) GH at a higher dose has been tested in pediatric burned subjects with minor adverse events, and b) the subjects will be monitored consistently.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
13
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
0.09% saline solution0.09% Saline SolutionDaily subcutaneous injections of 0.09% of saline solution will be administered, from one week prior to discharge until 9 months post-burn.
Growth HormoneSomatropinDaily subcutaneous injections of 0.05 mg/kg/day of Growth Hormone \[somatropin, Genotropin, Pfizer, New York, NY\] will be administered, from one week prior to discharge until 9 months post-burn.
Primary Outcome Measures
NameTimeMethod
Lean body mass24 months post burn-injury

Dual-Energy X-ray Absorptiometry (DEXA) measured in grams

Secondary Outcome Measures
NameTimeMethod
Change in Muscle strength ( total work)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

resistance testing muscle strength (Biodex isokinetic dynamometer)

Change in Muscle strength (average power)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

resistance testing muscle strength (assessed by Biodex isokinetic dynamometer)

Change in Muscle grip strength (maximum power)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

testing muscle strength (assessed by hand dynamometer)

Change in Resting energy expenditure (REE)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Indirect calorimetry

Change in resting heart rateDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

electrocardiogram (EKG) readings

Change in liver sizeDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Ultrasound readings

Change in cardiac outputDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Echocardiogram readings

Change in bone mineral contentDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Dual-Energy X-ray Absorptiometry (DEXA)

Change in forced expiratory volume in one second (FEV1)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in vital capacity (VC)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in total lung capacity (TLC)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in residual volume (RV)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in Muscle strength (peak torque)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

resistance testing muscle strength (assessed by Biodex isokinetic dynamometer)

Change in respiratory fatigueDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Six-minute walk test

Change in Forced vital capacity (FVC)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in Muscle endurance (maximum power)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

testing muscle endurance (assessed by Bruce treadmill test)

Change in cardiac stroke volumeDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

echocardiogram readings

Change in respiratory quotientDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Indirect calorimetry

Change in Total body fatDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Dual-Energy X-ray Absorptiometry (DEXA)

Change in percentage of total body fatDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Dual-Energy X-ray Absorptiometry (DEXA)

Change in Maximal oxygen consumptionDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Six-minute walk test

Hypertrophic scar developmentDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Vancouver Scar Scale

Change in bone mineral densityDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Dual-Energy X-ray Absorptiometry (DEXA)

Change in leg fatigueDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Six-minute walk test

Change in Muscle protein synthesis rateDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Deuterium water

Change in FEV1/FVC ratio expressed as a percentage (FEV1/FVC%)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in forced expiratory flow rate between 27-75% of the FVC (FEF25-75)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in functional residual capacity (FRC)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in lung diffusion capacity (DLCO)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in maximum voluntary ventilation (MVV)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in peak expiratory flow (PEF)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Pulmonary function test

Change in Molecular biomarkers of oxidative and nitrosative stress (isoprostanes, asymmetric dimethylarginine)Discharge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Urine analysis

Changes in quality of lifeDischarge (usually 30 days post burn) and 6, 12, 18, and 24 months after burn injury

Questionnaires

Trial Locations

Locations (1)

University of Texas Medical Branch

🇺🇸

Galveston, Texas, United States

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