Identification of Specific Molecular Signatures by Capture-HPV and Next-generation Sequencing in HPV-induced Oropharyngeal Cancers
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Oropharyngeal Cancer
- Sponsor
- Assistance Publique - Hôpitaux de Paris
- Enrollment
- 66
- Locations
- 1
- Primary Endpoint
- Description the HPV molecular signatures identified by the Capture-HPV technique and comparison of these signatures with those already described in the classification described in the Holmes et al. in the cervix
- Status
- Completed
- Last Updated
- 4 years ago
Overview
Brief Summary
The aim of this study is to identify HPV molecular signature in head and neck cancer to establish a new classification for positive human papillomavirus oropharyngeal tumor
Detailed Description
OROPAP is a monocentric exploratory study with retrospective inclusion of patients. The retrospective study focus on patients who have had surgery for HPV-positive oropharyngeal tumors whose survival is at least 2 year. Frozen and or FFPE biopsies of these tumors and frozen blood samples are available via the HEGP biological resources platform. The HPV molecular signatures will be identified by the capture-HPV technique. For each patient, clinical-anatomo-pathological data such as tumor size, tumor stage, presence or absence of metastasis, histological stage, different treatment lines and 2-years survival data will be collected through the use of data warehouse available on the HEGP.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Patients with HPV + oropharyngeal cancer
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Description the HPV molecular signatures identified by the Capture-HPV technique and comparison of these signatures with those already described in the classification described in the Holmes et al. in the cervix
Time Frame: 18 months
Establish a new classification based on HPV molecular signature for positive human papillomavirus oropharyngeal tumor
Secondary Outcomes
- Correlate identified HPV molecular signature with clinical-anatomo-pathological data.(18 months)
- Description of new HPV viral variants based on complete HPV genome sequencec obtained by Capture-HPV coupled with NGS(18 months)
- Quantification of HPV circulating tumoral DNA in peripheral blood by droplet based digital PCR(18 months)
- Number of Death of any cause, or eventual relapse(18 months)
- Ratio of the number of copies of viral genes to the number of cells(18 months)
- Precise description of HPV insertion localisation in human genome when HPV is integrated(18 months)