The Acute Effect of Instant Coffee in Different Forms on Plasma Metabolites
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 1
- 主要终点
- Caffeine and polyphenol metabolites
研究概览
简要总结
This study is designed to evaluate the acute effect of consuming a cup of instant coffee on circulating metabolites and to evaluate whether ingestion of instant coffee in the form of a capsule has a similar effect on circulating metabolites as compared to the conventionally prepared beverage.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 21 Years 至 64 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy individuals aged 21-64 years old of Chinese ethnicity and are physically-abled, can participate in the research, and are able to understand English
排除标准
- •Pregnant or breastfeeding
- •Certain medications including Zanaflex, Sirasalud, Ketanest S, Spravato and Ephedrine Sulfate
- •Any pre-existing medical conditions, depression, anxiety, caffeine/lactose intolerant, fear of needles and/or blood disorders, allergy to coffee or bovine capsules
- •Existing dietary restrictions which makes them unable to consume non-halal certified bovine gelatin (the capsule encasing the coffee powder)
- •Recent history of major surgical operations in the past year
- •Past or current user of oral/systemic hormone treatment or therapies
- •Taken antibiotics in the last 3 months
- •Taking nutritional supplements (that contains coffee/tea-related compounds naturally found in other plants)
- •Not willing to travel to the University for the study
- •Unable to commit to completing both study visits
研究组 & 干预措施
Instant coffee capsule/ coffee beverage
干预措施: Instant Coffee Capsule (Dietary Supplement)
Instant coffee capsule/ coffee beverage
干预措施: Instant Coffee Beverage (Dietary Supplement)
Instant coffee beverage/ coffee capsule
干预措施: Instant Coffee Capsule (Dietary Supplement)
Instant coffee beverage/ coffee capsule
干预措施: Instant Coffee Beverage (Dietary Supplement)
结局指标
主要结局
Caffeine and polyphenol metabolites
时间窗: The period between the two study visits ranged between 7 to 28 days.
Each participant underwent two interventions, and blood was collected pre- and post- each intervention. Blood was allowed to clot at room temperature. After 30-40 min, serum was separated from entire blood by centrifugation (3000g, 15 min, 4°C) and aliquoted into tubes. All samples were processed on the same day of collection. A total of 7 biomarker compounds (trigonelline, paraxanthine, theophylline, theobromine, caffeine, cyclo(Leu-Pro), and cyclo(Pro-Val)) were quantified using a targeted method developed in-house. The quantification method was optimized by LC retention elution, parent-daughter quantifier mass transitions, and varying MS cone and collision energies (in V) to obtain clear peak separation and height. Multiple Reaction Monitoring (MRM) in positive electrospray ionization mode was performed on a Waters Xevo TQ-S Triple Quadrupole Mass Spectrometer system.
次要结局
未报告次要终点
研究者
Seow Wei Jie
Assistant Professor
National University of Singapore