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Safety, Tolerability, Pharmacokinetics, and Preliminary Efficacy of SPL84 in Patients with Cystic Fibrosis

Phase 2
Recruiting
Conditions
Cystic Fibrosis
Interventions
Other: Placebo
Registration Number
NCT06429176
Lead Sponsor
SpliSense Ltd.
Brief Summary

The goal of this clinical trial is to learn if drug SPL84 is safe for adult patients with cystic fibrosis (CF). It will also learn if the drug works to treat works to treat CF with a specific mutation.

The purpose of this research study is to:

* test the safety and effectiveness of multiple doses of the study drug, SPL84

* test how multiple doses of the drug are processed by the body

Researchers will compare drug SPL84 to a placebo (a look-alike substance that contains no drug) to see if drug SPL84 is safe and if it works to treat CF.

Participants will:

Take drug SPL84 or a placebo by inhalation every week for 9 weeks months Visit the clinic approximately 14 times over 17.5 weeks for checkups and tests

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Diagnosis of CF and two CF causing mutations; 3849+10 Kb C->T mutation on one allele in the CF transmembrane conductance regulator (CFTR) gene (homozygote or compound heterozygote). Source documentation from a certified genetic laboratory is required.
  • Body mass index (BMI) of ≥ 17 kg/m2.
  • FEV1 40-90% predicted at screening.
  • Non-smokers or vapers for at least 180 days (6 months) prior to screening, per participant report.
Exclusion Criteria
  • Use of Kalydeco, Orkambi, Symdeko/Symkevi or Trikafta/Kaftrio within 30 days of first dose with study intervention.
  • Use of any investigational drug (other than SPL84) or device within 30 days of first dose with study intervention.
  • Use of systemic steroids over 3 consecutive months in the last 6 months prior to screening, or use of systemic steroids in the last month prior to screening. Use of inhaled steroids above 1 mg.
  • Use of CF medications, e.g. inhaled antibiotics, dornase alfa (Pulmozyme), hypertonic saline and physiotherapy should be on stable regimen for the period 28 days prior to screening; those participants taking inhaled antibiotics for prophylaxis must be on a stable regimen of these drugs for at least 90 days prior to first dose with study intervention.
  • Any acute infection including acute upper respiratory or lower respiratory infections, pulmonary exacerbation, changes in therapy for pulmonary disease, or any non CF-related illness which results in the initiation of any new therapy within 14 days prior to first dose with study intervention.
  • Hemoptysis of greater than 30 mL within 90 days prior to Day 1, or hospitalization for hemoptysis within 6 months of first dose with study intervention.
  • Liver disease characterized by clinically significant cirrhosis and/or documented portal hypertension.
  • History of any organ transplantation.
  • Documented coronavirus disease (COVID-19) infection within 4 weeks prior to dosing.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
SPL84SPL84-
PlaceboPlacebo-
Primary Outcome Measures
NameTimeMethod
Safety and Tolerability of SPL84 as evaluated by number of subjects with at least one treatment-related adverse event (AE) or serious adverse event (SAEs)Day 1 through Day 87

Incidence, nature, and severity of AEs and SAEs

Safety and Tolerability of SPL84 as assessed by number of participants with abnormal systolic and diastolic blood pressureDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal heart rateDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal respiratory rateDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal oximetryDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal sputum microbiology results resultsDay 1 through Day 87

Sputum microbiology will be performed with a microbiology based assay; organism growth will be identified.

Safety and Tolerability of SPL84 as assessed by number of participants with abnormal immunogenicity resultsDay 1 through Day 87

assessment of anti-SPL84 antibodies will be performed both in serum and sputum

Safety and Tolerability of SPL84 as assessed by number of participants with abnormal temperatureDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal biochemistry lab test resultsDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal urinalysis lab test resultsDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal hematology lab test resultsDay 1 through Day 87
Safety and Tolerability of SPL84 as assessed by number of participants with abnormal electrocardiogram (ECG) parametersDay 1 through Day 87

using an ECG machine that automatically calculates heart rate and measure PR, QRS, QT, and QTc intervals

Safety and Tolerability of SPL84 as assessed by number of participants with abnormal physical examination findingsDay 1 through Day 87

Complete physical examinations include general appearance, head, ears, eyes, nose, throat, thyroid, chest (heart, lungs), abdomen, skin, neurological, extremities, back, neck, musculoskeletal, and lymph nodes.

Safety and Tolerability of SPL84 as assessed by number of participants with abnormal pulmonary function tests resultsDay 1 through Day 87

Pulmonary function tests will be performed according to the American Thoracic Society (ATS)/European Respiratory Society (ERS) and forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), and forced mid-expiratory flow (FEF25-75) will be measured

Secondary Outcome Measures
NameTimeMethod
Characterization of PK of SPL84: Area under the curve to infinity (AUC0-∞)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87
Characterization of PK of SPL84: Apparent clearance (CL/F)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87
Preliminary efficacy of SPL84 as assessed by change from baseline in body weightDay 1 through Day 87
Characterization of pharmacokinetics (PK) of SPL84: maximum serum concentration (Cmax)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87
Preliminary efficacy of SPL84 as assessed by change from baseline in Cystic Fibrosis Questionnaire-Revised Respiratory Symptom ScoreDay 1 through Day 87

The score for is standardized on a 0- to 100-point scale on which higher scores represent a higher quality of life

Preliminary efficacy of SPL84 as assessed by change from baseline of antibiotic treatmentDay 1 through Day 87
Characterization of PK of SPL84: Time to Cmax (Tmax)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87
Characterization of PK of SPL84: terminal elimination half-life (t1/2)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87
Characterization of excretion of SPL84: concentration of SPL84 in urineDay 1 through Day 87
Preliminary efficacy of SPL84 as assessed by change from baseline in percent predicted FEV1Day 1 through Day 87

Pulmonary function tests will be performed according to the ATS/ERS

Characterization of PK of SPL84: Area under the curve to the final sample (AUC0-t)Predose and 15 and 30 minutes and 1, 2, 4, 6, 8, and 24 hours postdose on Day 1 and Day 57; predose on Days 8 and 29; Days 64 and 87

Trial Locations

Locations (2)

Boston Children'S Hospital

🇺🇸

Boston, Massachusetts, United States

Nationwide Children'S Hospital

🇺🇸

Columbus, Ohio, United States

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