Effects of Ischemic Postconditioning on Expression of Apoptosis-related MicroRNAs and Genes in Human Double Valve Replacement
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 8
- 试验地点
- 1
- 主要终点
- Change from Baseline in Cardiac MicroRNA Expression
研究概览
简要总结
- Cardiopulmonary bypass and cardioplegic arrest could regulate expression of microRNAs in patients undergoing double valve replacement (aortic and mitral).
- The modulation of myocardial microRNAs by cardiopulmonary bypass and cardioplegic arrest may be rescued by ischemic postconditioning.
- Downstream effectors would also be affected.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 20 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Consecutive eight patients with rheumatic heart valve disease undergoing elective double valve replacement(aortic and mitral)are considered for participation in this study.
排除标准
- •insulin-dependent diabetes mellitus
- •pulmonary, renal, or hepatic failure
- •infective valve disease
- •valve disease with coronary artery disease
- •hypertension
- •emergency and reoperations
- •received aspirin,corticosteroids, or statin preoperatively
- •received preoperative inotropic support
研究组 & 干预措施
CON
Patients undergoing double valve replacement (aortic and mitral).
干预措施: double valve replacement (Procedure)
CON
Patients undergoing double valve replacement (aortic and mitral).
干预措施: heart-lung machine (Device)
CON
Patients undergoing double valve replacement (aortic and mitral).
干预措施: Aortic cross-clamp (Device)
IPO
Patients undergoing double valve replacement (aortic and mitral) with ischemic postconditioning.
干预措施: ischemic postconditioning (Procedure)
IPO
Patients undergoing double valve replacement (aortic and mitral) with ischemic postconditioning.
干预措施: double valve replacement (Procedure)
IPO
Patients undergoing double valve replacement (aortic and mitral) with ischemic postconditioning.
干预措施: heart-lung machine (Device)
IPO
Patients undergoing double valve replacement (aortic and mitral) with ischemic postconditioning.
干预措施: Aortic cross-clamp (Device)
结局指标
主要结局
Change from Baseline in Cardiac MicroRNA Expression
时间窗: before cardiopulmonary bypass and at 5 min after aortic de-clamping
Samples were harvested from all the patients, respectively, before cardiopulmonary bypass and at 5 min after aortic de-clamping. Real-time quantitative stem-loop reverse transcription polymerase chain reaction assay was performed to detect the expression of microRNAs.
次要结局
- Change from Baseline in Downstream Gene Expression(before cardiopulmonary bypass and at 5 min after aortic de-clamping)
研究者
Luo Wanjun
Prof.
Central South University