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Study of the Impact of CD34+ Cell Dose on Absolute Lymphocyte Count Following High-Dose Therapy and Autologous Stem Cell Transplantation for Relapsed and Refractory Diffuse Large B-cell Lymphoma (DLBCL)

Phase 2
Active, not recruiting
Conditions
Refractory Diffuse Large B-cell Lymphoma (DLBCL)
Diffuse Large B-cell Lymphoma (DLBCL)
Relapsed Diffuse Large B-cell Lymphoma (DLBCL)
Interventions
Procedure: leukapheresis
Procedure: Autologous Stem Cell Transplantation
Registration Number
NCT02570542
Lead Sponsor
Memorial Sloan Kettering Cancer Center
Brief Summary

The purpose of this study is to study the impact of stem cell dose on outcome after autologous transplant.

Detailed Description

Following enrollment, patients will be CD34+ stem cell mobilized at the discretion of the treating attending physician with the plerixafor for the achievement of \>6 x10\^6 CD34+ cells/kg. The patients that fail to mobilize \>6 x10\^6 CD34+ cells/kg will not be randomized and will subsequently be followed for disease progression and overall survival.. Patients with \>6 x10\^6 CD34+ cells/kg cryopreserved on study will be admitted to the hospital for planned ASCT. Patients will be randomly infused with either 3-4 x 10\^6 CD34+ stem cells/kg or 6-8 x10\^6 CD34+ stem cells/kg on d0 per study randomization. The cell dose ranges within the two groups allows variability within aliquots of cells at the time of cryopreservation. Patients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.

Recruitment & Eligibility

Status
ACTIVE_NOT_RECRUITING
Sex
All
Target Recruitment
59
Inclusion Criteria
  • Age ≥ 18 years old

  • Diagnosed with relapsed or refractory de novo DLBCL or follicular lymphoma transformed to DLBCL to one previous line of anthracycline-containing chemotherapy

  • KPS ≥ 70

  • Complete or partial response by IWG Working Group or ICML Criteria to maximum of one salvage line of chemotherapy without pre-HDT/ASCT salvage radiotherapy.

  • Eligible for high-dose therapy and autologous stem-cell rescue

    • Serum creatinine ≤ 1.5 mg/dL, or if creatinine >1.5 mg/dL, calculated creatinine clearance of ≥50 mL/min by 24 hour creatinine clearance or CKD-EPI.
    • Last cycle of most recent salvage therapy within 8 weeks of enrollment
  • Total bilirubin < 2.0 mg/dL

    o If Gilbert"s disease is suspected and total bilirubin > 2.0 mg/dL, direct bilirubin must be < 2.0 mg/dL

  • Females of childbearing potential and males must agree to use an acceptable form of contraception per institutional practices.

Exclusion Criteria
  • Disease progression by IWG Working Group or ICML Criteria since last therapy
  • Prior autologous or allogeneic stem cell transplantation
  • HIV infection
  • Comorbid condition(s) which, in the opinion of the attending physician and/or MSKCC Principal Investigator, will preclude stem cell mobilization and/or high-dose therapy with autologous stem cell rescue
  • Treatment plan that includes post-transplant maintenance therapy
  • Salvage therapy that includes involved field radiotherapy

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
3-4 x 10^6 CD34+ stem cells/kgleukapheresisPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
3-4 x 10^6 CD34+ stem cells/kgAutologous Stem Cell TransplantationPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
3-4 x 10^6 CD34+ stem cells/kgcarmustine, etoposide, cytarabine, melphalanPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
6-8 x10^6 CD34+ stem cells/kgleukapheresisPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
6-8 x10^6 CD34+ stem cells/kgcarmustine, etoposide, cytarabine, melphalanPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
6-8 x10^6 CD34+ stem cells/kgAutologous Stem Cell TransplantationPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
3-4 x 10^6 CD34+ stem cells/kgPlerixaforPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
6-8 x10^6 CD34+ stem cells/kgPlerixaforPatients will receive standard supportive measures (including: growth factor support post-HDT/ASCT, antimicrobial prophylaxis, red blood cell and platelet transfusion and treatment for neutropenic fever) as per institutional guideline practices.
Primary Outcome Measures
NameTimeMethod
progression-free survival (PFS)at +/- 2 weeks

equals date of progression/death - date of Autologous Stem Cell Transplantation

Secondary Outcome Measures
NameTimeMethod
the impact of CD34+ cell dose on lymphocyte subset recoveryday 15

(ALC15) post HDT-ASCT Accordingly, each subject will be classified as a responder (i.e., recovery) or non-responder.

Trial Locations

Locations (14)

Memorial Sloan Kettering Commack (Consent and Follow-up Only)

🇺🇸

Commack, New York, United States

Memorial Sloan Kettering Westchester

🇺🇸

Harrison, New York, United States

Columbia University

🇺🇸

New York, New York, United States

Northwell Health (Data collection only)

🇺🇸

Manhasset, New York, United States

Memorial Sloan Kettering Cancer Center

🇺🇸

New York, New York, United States

Memorial Sloan Kettering Nassau (Consent and Follow up Only)

🇺🇸

Uniondale, New York, United States

Tennessee Oncology

🇺🇸

Nashville, Tennessee, United States

Texas Transplant Institute

🇺🇸

San Antonio, Texas, United States

Medical College of Wisconsin

🇺🇸

Milwaukee, Wisconsin, United States

Memorial Sloan Kettering Basking Ridge (Consent and Follow-Up Only)

🇺🇸

Basking Ridge, New Jersey, United States

University of Nebraska Medical Center

🇺🇸

Omaha, Nebraska, United States

Memorial Sloan Kettering Bergen (Consent and Follow Up Only)

🇺🇸

Montvale, New Jersey, United States

Memorial Sloan Kettering Monmouth (Consent and Follow up Only)

🇺🇸

Middletown, New Jersey, United States

University of Rochester Medical Center

🇺🇸

Rochester, New York, United States

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