Gender Dependent Difference in Lipemia After 6 x OFTT in Young Healthy Subjects

Not Applicable

Completed

• Conditions: Hyperlipidemia

• Registration Number: NCT02506920
• Lead Sponsor: Herning Hospital

Brief Summary

The investigators wanted to study the postprandial lipemia after six oral fat tolerance test with varying amounts of saturated fat in young healthy subjects.

Detailed Description

With six different types of butter added in random order to potatoes the investigators studied postprandial lipemia (PPL), lipoprotein, glucose, and insulin increments for eight hours in 14 young, lean, healthy students, seven of each gender.

The six, isocaloric meals contained 55.1 g carbohydrate, 5.8 g protein, 82.3 g fat, and 4177 kJ. The potatoes, without skin, were boiled in 20 minutes in excess of water with 5 g of salt added, were blended in 4 minutes and served hot. The meals were ingested over 10 minutes, with 250 ml tap water. The following 8 hours the participants stayed at the clinic to watch television, read or take a short walk of 10 minutes.

Blood samples were drawn at -15, 0, 30, 60, 90,120,180,240,300,360, 420 and 480 minutes after the meals and processed after each drawing. After blood collection, plasma was immediately separated by centrifugation at 3000 rpm for 10 minutes at 4°C.

The area under the curve (AUC) were determined for TG, HDL, LDL, total cholesterol, FFA, insulin, and glucose concentrations by the trapezoid method. Multiple regression was performed to adjust for age, gender, meals, and BMI to the variance of postprandial responses. The difference between meals were tested with ANOVA. If p\<0.05 in ANOVA, post-hoc-testing with Student-Newman-Keul's test was performed to identify, which meal was significantly different from the other, and to minimize the risk of error by multiple testing.

Study Timeline

• Study Start: 1997-01
• Primary Completion: 1997-04
• Study Completion: 1997-12
• Study First Submitted: 2015-06-26
• Status Verified: 2015-07
• Study First Submitted QC: 2015-07-22
• Last Update Submitted: 2015-07-22
• Study First Posted: 2015-07-23
• Last Update Posted: 2015-07-23

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 14

Inclusion Criteria

• Accept healthy volunteers

Exclusion Criteria

• hypercholesterolemia,
• hypertension,
• abuse of drugs or alcohol,
• known kidney, liver, heart and metabolic disease
• hormonal therapies including oral contraceptives

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Postprandial free fatty acids	Eight hours	Free fatty acids measured in micro equivalents per liter
Postprandial cholesterol	Eight hours	Cholesterols measured in milli mol per liter

Secondary Outcome Measures

Name	Time	Method
postprandial insulin	Eight hours	Insulin measured in pico mol per liter
Postprandial glucose	Eight hours	Glucose measured in milli mol per liter