The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Other: Postabsorptive; Other: Postprandial; Procedure: Immobilisation

• Registration Number: NCT03934632
• Lead Sponsor: University of Exeter

Brief Summary

The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-04-15
• Study First Submitted: 2019-04-16
• Study First Submitted QC: 2019-04-29
• Study First Posted: 2019-05-02
• Primary Completion: 2020-03-17
• Study Completion: 2023-01-09
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-21
• Last Update Posted: 2023-03-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 24

Inclusion Criteria

• Male
• 18-40 years of age
• Body mass index between 18.5 and 30

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Exclusion Criteria

• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism)
• A personal or family history of epilepsy, seizures or schizophrenia
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements
• Allergy to lidocaine
• Allergy to one or multiple amino acids
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches.
• Having received or ingested a stable isotope tracer in the past

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Postabsorptive	Postabsorptive	Saline infusion to mimic postabsorptive circulating amino acid concentrations
Postabsorptive	Immobilisation	Saline infusion to mimic postabsorptive circulating amino acid concentrations
Postprandial	Postprandial	Amino acid infusion to mimic postprandial circulating amino acid concentrations
Postprandial	Immobilisation	Amino acid infusion to mimic postprandial circulating amino acid concentrations

Primary Outcome Measures

Name	Time	Method
Muscle protein breakdown rate	1 hour	Muscle protein breakdown rate (FBR, measured in %/h) during 1 hour of saline or amino acid infusion

Secondary Outcome Measures

Name	Time	Method
Muscle protein breakdown rate	3 hours	Muscle protein breakdown rate (FBR, measured in %/h) during 3 hours of saline or amino acid infusion
Muscle protein synthesis rate	3 hours	Muscle protein synthesis rate (FSR, measured in %/h) during 3 hours of saline or amino acid infusion measured using a D5 phenylalanine infusion

Trial Locations

Locations (1)

University of Exeter; 🇬🇧 Exeter, Devon, United Kingdom