The Impact of Leg Immobilization on Postabsorptive and Postprandial Muscle Protein Breakdown in Healthy Young Males

Not Applicable

Completed

• Conditions: Healthy

• Registration Number: NCT03934632
• Lead Sponsor: University of Exeter

Brief Summary

The present study will seek the quantify the simultaneous muscle protein synthesis and breakdown response with and without amino acid provision in humans following 2 days of immobilisation.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-04-15
• Study First Submitted: 2019-04-16
• Study First Submitted QC: 2019-04-29
• Study First Posted: 2019-05-02
• Primary Completion: 2020-03-17
• Study Completion: 2023-01-09
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-21
• Last Update Posted: 2023-03-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 24

Inclusion Criteria

• Male
• 18-40 years of age
• Body mass index between 18.5 and 30

Exclusion Criteria

• Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes)
• Any diagnosed cardiovascular disease (e.g. deep vein thrombosis) or hypertension
• Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism)
• A personal or family history of epilepsy, seizures or schizophrenia
• Any known disorders in muscle metabolism
• Regular use of nutritional supplements
• Allergy to lidocaine
• Allergy to one or multiple amino acids
• Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture) as these could be exacerbated by the intervention e.g. unilateral leg immobilisation, or mean the participant is unable to use crutches.
• Having received or ingested a stable isotope tracer in the past

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Muscle protein breakdown rate	1 hour	Muscle protein breakdown rate (FBR, measured in %/h) during 1 hour of saline or amino acid infusion

Secondary Outcome Measures

Name	Time	Method
Muscle protein breakdown rate	3 hours	Muscle protein breakdown rate (FBR, measured in %/h) during 3 hours of saline or amino acid infusion
Muscle protein synthesis rate	3 hours	Muscle protein synthesis rate (FSR, measured in %/h) during 3 hours of saline or amino acid infusion measured using a D5 phenylalanine infusion

Trial Locations

Locations (1)

University of Exeter; 🇬🇧 Exeter, Devon, United Kingdom