Bioavailability of Carotenoids and Flavonoids From Fresh Oranges and Orange Juice.

Not Applicable

• Conditions: Healthy
• Interventions: Other: Fresh orange fruit and pasteurized fruit juice

• Registration Number: NCT02380144
• Lead Sponsor: University of Hohenheim

Brief Summary

The study was designed as a randomized 2-way cross-over study to assess the bioavailability of carotenoids and flavonoids from sweet oranges and orange juice. Volunteers followed a wash-out period for 2 weeks prior to eating either 400 g of fresh oranges or 719 g of orange juice. The amount of test food was adjusted to deliver an equal amount of β-cryptoxanthin (740 µg). After a consecutive wash-out period of 2 weeks, the procedure was repeated and the volunteers ingested the complementary test food. Blood samples were collected at 8 time points up to 9.5 h after test meal consumption. Additionally, 24 h-urine was collected to evaluate flavonoid recovery.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-01
• Primary Completion: 2014-05
• Study First Submitted: 2015-02-23
• Study First Submitted QC: 2015-03-04
• Study First Posted: 2015-03-05
• Status Verified: 2016-10
• Last Update Submitted: 2016-10-30
• Last Update Posted: 2016-11-01
• Study Completion: 2017-07

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• Healthy adult men and women
• BMI: 17-60
• Age: 18-65

Exclusion Criteria

• Women who are lactating or pregnant or plan to be pregnant
• Individuals using tobacco products
• Individuals with metabolic or malabsorption disorders
• Individuals with a history of cancer
• Individuals with a history of liver insufficiency
• Individuals with a history of chronic and lipid metabolism-related diseases
• Individuals with an allergy to oranges
• Obese individuals (BMI > 30)

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Orange Fruit - Orange Juice	Fresh orange fruit and pasteurized fruit juice	Sequence of test foods during the intervention period: 1st: Orange fruit; 2nd: Orange juice
Orange Juice - Orange Fruit	Fresh orange fruit and pasteurized fruit juice	Sequence of test foods during the intervention period: 1st: Orange juice; 2nd: Orange fruit

Primary Outcome Measures

Name	Time	Method
Mean AUC (nmol/L*9.5 h) of beta-cryptoxanthin concentration in blood plasma chylomicrons after ingestion of fresh oranges and orange juice, respectively.	0 - 9.5 h after each intervention

Secondary Outcome Measures

Name	Time	Method
Mean hesperetin and naringenin concentration (µmol/L) in 24 h-urine after ingestion of fresh oranges and orange juice, respectively.	0 - 24 h after each intervention
Mean AUC of hesperidin- and narirutin-metabolite concentration in blood plasma after ingestion of fresh oranges and orange juice, respectively.	0 - 9.5 h after each intervention

Trial Locations

Locations (1)

Chair of Plant Foodstuff Technology and Analysis (150d), University of Hohenheim; 🇩🇪 Stuttgart, Baden-Württemberg, Germany