Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 6
- 主要终点
- Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis
研究概览
简要总结
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.
详细描述
OBJECTIVES:
- To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
- To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
- To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
- To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis
Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis
Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation
Role of microRNA-34a and microRNA-194 in myeloid cell proliferation
次要结局
未报告次要终点