Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia
- Conditions
- Leukemia
- Registration Number
- NCT01057199
- Lead Sponsor
- Children's Oncology Group
- Brief Summary
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.
- Detailed Description
OBJECTIVES:
* To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
* To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation.
* To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
* To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 6
Not provided
Not provided
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Downregulation of E2F3 by microRNA-34a and microRNA-194 during granulopoiesis Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis Relationship between overexpression of microRNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations and granulocytic differentiation Role of microRNA-34a and microRNA-194 in myeloid cell proliferation
- Secondary Outcome Measures
Name Time Method