Effect of Nicotinamide Riboside and Pterostilbene Supplementation on Muscle Regeneration in Elderly Humans - A Randomized, Placebo-controlled, Clinical Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 32
- 试验地点
- 2
- 主要终点
- Presence of Satellite Cells
研究概览
简要总结
Successful skeletal muscle regeneration depends on a functional pool of muscle stem cells, termed satellite cells (SC). SC are in a quiescent state throughout adulthood, but undergo multiple cycles of proliferation and self-renewal in response to muscle damage. During aging, there is a loss of SC quiescence, and SC more readily enter an ageing-state impairing their function.
Animal studies have revealed a common denominator for increasing SC function and activity, namely Sirtuin activation. Natural stimulators of Sirtuins includes Nicotinamide Riboside (NR) (a Nicotinamide adenine dinucleotide (NAD+) precursor) and the polyphenol Pterostilbene (PT). In this study, we aim to investigate if NR+PT supplementation will promote skeletal muscle regeneration after muscle damage in elderly humans by enhanced recruitment of SC.
详细描述
14 days after start of placebo or NR/PT (500/100 mg twice daily) supplementation a muscle damage will induced in the m. vastus lat. by electric stimulation combined with eccentric work in a dynamometer. Muscle biopsies will be collected at 2 hours, 2, 8 and 30 days post damage and placebo or NR/PT supplementation will continue for the whole study period (45 days).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 55 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Written signed consent
- •Age: 55-80
- •BMI: 20-28 (kg/(m2))
- •Non-smoker
排除标准
- •Endocrine disease, neurological or muscle disease
- •Other severe disease
- •High daily activity level (>30 min / day)
结局指标
主要结局
Presence of Satellite Cells
时间窗: Change from baseline up to 45 days
quantified by immunohistochemistry in muscle biopsies
次要结局
- Presence of damaged muscle fibers(Change from baseline up to 45 days)
- Lipid accumulation in skeletal muscle tissue and liver(Change from baseline to 8 days)
- Activation of Satellite cells(Change from baseline up to 45 days)
- Presence of macrophages(Change from baseline up to 45 days)
- Presence of Fibro/Adipogenic Progenitors(Change from baseline up to 45 days)
- Presence of muscle regenerative fibers(Change from baseline up to 45 days)
- Presence of autophagy in relation to muscle damage(Change from baseline up to 45 days)
- Blood glucose response in relation to muscle damage(Change from baseline to 8 days)
- Body composition (lean body mass and fat body mass)(Change from baseline to 45 days)
- Muscle strength(Change from baseline up to 45 days)