Nutrient Supplementation Influences Mitochondria Adaptation

Not Applicable

Completed

• Conditions: Nutrition; Performance; Protein
• Interventions: Dietary Supplement: CARBOHDYRATE; Dietary Supplement: PROTEIN-CARBOHYDRATE

• Registration Number: NCT03561337
• Lead Sponsor: University of Aarhus

Brief Summary

Twenty-four well trained runners (VO2max 60.73.7 ml O2 Kg-1 min1) completed a six week block randomized controlled intervention period. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO- CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 0.1g carbohydrate kg-1) after each exercise session. The CHO group ingested energy matched carbohydrate. Post-absorptive muscle biopsies were obtained pre and post intervention for analysis HAD, CS and PGC-1. Subjects completed two physical tests 6 km performance test (pre, week 1 and week 6) and aVo2max test (pre and post intervention).

Detailed Description

Not available

Study Timeline

• Study Start: 2013-10-01
• Primary Completion: 2014-02-01
• Study Completion: 2014-06-01
• Study First Submitted: 2017-12-01
• Study First Submitted QC: 2018-06-07
• Study First Posted: 2018-06-19
• Status Verified: 2020-05
• Last Update Submitted: 2020-05-12
• Last Update Posted: 2020-05-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

1. maximal oxygen uptake (VO2max) of >50ml O2 kg-1 min-1 for women and >55ml O2 kg-1 min-1 for men,
2. running at least 3 times a week and
3. running being the primarily training form

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Exclusion Criteria

1. use of medicine
2. diagnosed metabolic diseases
3. injuries which hindered running and body mass index (BMI) < 25 kg m-2

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
CARBOHYDRATE	CARBOHDYRATE	Intervention groups receiving carbohydrate supplement
PROTEIN-CARBOHYDRATE	PROTEIN-CARBOHYDRATE	Intervention group receiving protein and carbohydrate supplement

Primary Outcome Measures

Name	Time	Method
Maximal oxygen uptake	Change from week 1 to week 6	maximal oxygen uptake (L O2/min)

Secondary Outcome Measures

Name	Time	Method
VDAC	Change from week 1 to week 6	Mitochondria target measured by western blotting
CytC	Change from week 1 to week 6	Mitochondria enzyme measured by western blotting
COX-IV	Change from week 1 to week 6	Mitochondria target measured by western blotting
HSP60	Change from week 1 to week 6	Mitochondria target measured by western blotting
CD36	Change from week 1 to week 6	Fat transporter measured by western blotting
Performance	Change from week 1 to week 6	6 km trial test

Trial Locations

Locations (2)

Aarhus University, Department for Public Health, Section for Sport Science; 🇩🇰 Aarhus, Denmark

Sport Science, Department of Public Health, Aarhus University; 🇩🇰 Aarhus, Denmark