Adipose tissue oxygen tension: adipose tissue depot differences and functional consequences

Completed

Conditions: adult-onset diabetes
type 2 diabetes mellitus
10018424

Registration Number: NL-OMON40421

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 14

Inclusion Criteria

- Woman with overweight/obesity (body mass index >= 28 kg/m2) and a disturbed glucose metabolism (IFG: 6.1-7.0 mmol/l (or 5.6-7.0 mmol/l and a family history of diabetes) and/or IGT: 2h plasma glucose during OGTT 7.8-11.1 mmol/l)
- postmenopausal
- Age: 40-65
- Non smoking
- A stable bodyweight for at least 3 months (no change in bodyweight: < 3kg)

Exclusion Criteria

- diabetes mellitus
- cardiovascular diseases
- cancer
- asthma or bronchitis
- liver and/or kidney disease (determined based on ALAT and creatinine levels, respectively)
- a disease with a life expectancy < 5 years (will be questioned for each individual)
- alcohol and/or drug abuse (alcohol consumption > 15 units/week)
- plans to lose weight (subjects will be asked if they have weight loss plans (e.g. to increase their physical activity level or change diet): a positive answer will lead to exclusion)
- participation in sport activities for more than 3 hours/week
- Usage of higher doses anti-oxidants (vitamin A, C, E, beta-carteen; standard multi-vitamin supplements are allowed when the concentrations are less than: 800ug/day Vit A, 60mg/day Vit C, 10mg/day Vit E and 400ug/day beta-caroteen)
- use of medication with an effect on the glucose metabolism or inflammation
- not able to understand the subject information
- smoking

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Differences in oxygen tension and further effects for both adipose tissue<br /><br>depots in obese, insulin resistant woman will be investigated. We will perform<br /><br>our experiments in the abdominal and femoral adipose tissue depots and also<br /><br>monitor the blood flow in both depots. Next to this we will also determine the<br /><br>local inflammation.<br /><br><br /><br>The primary parameter is:<br /><br>- oxygen tension in adipose tissue</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secundary parameters are:<br /><br>- adipose tissue blood flow<br /><br>- insulin sensitivity<br /><br>- inflammation markers in adipose tissue (IL-6, TNF-alfa, CD68, leptin,<br /><br>adiponectin, MCP-1, MIF1, PPAR-gamma, CD34 mRNA expressie)<br /><br>- bodyweight, body mass index (BMI) and body composition<br /><br>- whole body adipose tissue distribution (DEXA scan)<br /><br>- adipocyte size<br /><br>- plasma glucose concentration<br /><br>- plasma insulin concentration<br /><br>- glucose tolerance<br /><br>- FFA, triacylglycerol, glycerol concentrations<br /><br>- bloodpressure<br /><br><br /><br>We will also isolate cells from the biopsies and expose them to different<br /><br>concentrations oxygen. With this experiment we will investigate the effects of<br /><br>oxygen tension on adipocyte metabolism and inflammation.</p><br>