Contribution of a defective adipose tissue and skeletal muscle lipolysis to diacylglycerol-mediated insulin resistance in obesity and type 2 diabetes

Recruiting

• Conditions: Obesitas; type 2 diabetes; 10018424

• Registration Number: NL-OMON34508
• Lead Sponsor: niversiteit Maastricht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 6 May 2024

Recruitment & Eligibility

• Status: Recruiting
• Sex: Not specified
• Target Recruitment: 30

Inclusion Criteria

- Lean men (n=10; normoglycemic BMI 20-25 kg/m2)
- Obese men (n=10 insulin resistant and n=10 insulin sensitive based on HOMA; BMI 30-35 kg/m2)
- Aged 45-65 years
- Caucasian
- Normal blood pressure (systolic blood pressure 100-140 mmHg, diastolic blood pressure 60-90 mmHg)
- Weight stable in last 3 months (± 2kg).

Exclusion Criteria

- Smokers
- People with intensive fitness training, eg. athletes (>= 3 per week >= 1 hour training)
- Serious pulmonary, cardiovascular, hepatic or renal disease
- History of cardiovascular disease
- Bleeding disorders
- Current use of medication interfering with study endpoints/hypotheses (e.g. beta-blockers)
- Not to be able to understand the study information
- Subjects on a special diet or vegetarian
- Blood donation 2 months prior to the study and during the study
- Participation in other studies

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main study parameter is the effect of selective knockdown on glycerol<br /><br>release in the medium (as marker of lipolysis), TAG and DAG lipase activity,<br /><br>and lipid content in human adipocyte and myocyte cell cultures. </p><br>

Secondary Outcome Measures

Name	Time	Method
<p>As secondary endpoints, differences in localization and trafficking of<br /><br>lipolytic proteins will be investigated in adipocytes derived from obese<br /><br>insulin resistant versus insulin sensitive men. </p><br>